Transcriptomics,Genomics

Dataset Information

33

Gut microbiota directs PPARγ-driven reprogramming of the liver circadian clock by nutritional challenge


ABSTRACT: The liver circadian clock is reprogrammed by nutritional challenge through the rewiring of specific transcriptional pathways. As the gut microbiota is tightly connected to host metabolism, whose coordination is governed by the circadian clock, we explored whether gut microbes influence circadian homeostasis and how they distally control the peripheral clock in the liver. Using fecal transplant procedures we reveal that, in response to high fat diet, the gut microbiota drives PPARγ-mediated activation of newly oscillatory transcriptional programs in the liver. Moreover, antibiotics treatment prevents PPARγ-driven transcription in the liver, underscoring the essential role of gut microbes in clock reprogramming and hepatic circadian homeostasis. Thus, a specific molecular signature characterizes the influence of the gut microbiome in the liver, leading to the transcriptional rewiring of hepatic metabolism. We used microarray to quantify the tissue specific expression level of circadian genes in terms of total RNA. Overall design: Microarray experiments were conducted to collect tissue specific, time specific samples from mice under different fecal transplant conditions, i.e. accepting fecal transplant from donors under certain diet conditions while being fed normal diet. Results compared to previous published works.

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Yu Liu  

PROVIDER: GSE82250 | GEO | 2016-06-04

SECONDARY ACCESSION(S): PRJNA324436

REPOSITORIES: GEO

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Publications

Gut microbiota directs PPARγ-driven reprogramming of the liver circadian clock by nutritional challenge.

Murakami Mari M   Tognini Paola P   Liu Yu Y   Eckel-Mahan Kristin L KL   Baldi Pierre P   Sassone-Corsi Paolo P  

EMBO reports 20160714 9


The liver circadian clock is reprogrammed by nutritional challenge through the rewiring of specific transcriptional pathways. As the gut microbiota is tightly connected to host metabolism, whose coordination is governed by the circadian clock, we explored whether gut microbes influence circadian homeostasis and how they distally control the peripheral clock in the liver. Using fecal transplant procedures we reveal that, in response to high-fat diet, the gut microbiota drives PPARγ-mediated activ  ...[more]

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