Multiple hierarchies of Polycomb targeting to Drosophila genome (Part 3)
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE83247 | GEO | 2016/09/15
SECONDARY ACCESSION(S): PRJNA325406
REPOSITORIES: GEO
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Project description:Polycomb group (PcG) proteins dynamically define cellular identities through epigenetic repression of key developmental genes. PcG target gene repression can be stabilized through the interaction in the nucleus at PcG foci. Here, we report the results of a high-resolution microscopy genome-wide RNAi screen that identifies 129 genes that regulate the nuclear organization of Pc foci. Candidate genes include PcG components and chromatin factors, as well as many novel protein-modifying enzymes, including components of the SUMOylation pathway. In the absence of SUMO Pc foci coagulate into larger aggregates. Conversely, loss of function of the SUMO peptidase velo disperses Pc foci. Moreover, SUMO and velo colocalize with PcG proteins at PREs and Pc SUMOylation affects its chromatin targeting, suggesting that the dynamic regulation of Pc SUMOylation regulates PcG-mediated silencing by modulating the kinetics of Pc binding to chromatin as well as its ability to form Polycomb foci.
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Project description:We have studied the linkage between the binding of Polycomb Group (PcG) proteins and the developmental regulation of gene expression using whole-genome mapping to identify sites bound by the PcG proteins, Polycomb (Pc) and Pleiohomeotic (Pho), in the Drosophila embryo and in a more restricted tissue, the imaginal discs of the third thoracic segment. Our data provide considerable support for the idea that Pho is a general component of the maintenance machinery since the majority of Pc targets are also associated with Pho binding. We find, in general, considerable developmental stability of Pc and Pho binding at target genes and observe that Pc/Pho binding can be associated with both expressed and inactive genes. In particular, at the Hox complexes both active and inactive genes have significant Pc and Pho binding; however, in comparison to inactive genes the active Hox genes show reduced and altered binding profiles. Keywords: ChIP-on-chip, tissue-specific binding
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