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Next Generation Sequencing of liver and subcutaneous fat tissues obtained from obese subjects

ABSTRACT: Patients had low calorie diet weight reduction run in prior to the day of surgery. The human liver and subcutaneous fat tissue samples were obtained from 12 obese subjects undergoing bariatric surgery and then used for the mRNA expression analyses.

ORGANISM(S): Homo sapiens

PROVIDER: GSE83322 | GEO | 2016/06/15

SECONDARY ACCESSION(S): PRJNA325592

REPOSITORIES: GEO

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Next Generation Sequencing of liver and subcutaneous fat tissues obtained from obese subjects

Project description:Patients had low calorie diet weight reduction run in prior to the day of surgery. The human liver and subcutaneous fat tissue samples were obtained from 12 obese subjects undergoing bariatric surgery and then used for the mRNA expression analyses. mRNA profiles of human liver and subcutaneous fat tissue samples were generated by RNA sequencing using Illumina HiSeq 2500. This dataset is part of the TransQST collection.

2016-06-15 | E-GEOD-83322 | biostudies-arrayexpress

Transcriptional profiling of subcutaneous adipose tissue of morbidly obese subjects before and after 2 years of bariatric surgery.

Project description:Transcriptional profiling of subcutaneous adipose tissue before and after 2 years of bariatric surgery. This type of surgery produce a masive weight loss in morbidly obese subjects, and improve the comorbidities associated to obesity. Goal was to determine the effects of bariatric surgery on the gene expression of subcutaneous adipose tissue.

2017-03-16 | GSE66921 | GEO

Individualized analysis of subcutaneous and omental abdominal adipose tissue from non-obese and obese subjects

Project description:Individualized analysis through expression profiling of 20,000 probes in 28 tissue samples evaluated in subcutaneous and omental adipose tissue obtained during surgical intervention in non-obese and obese patients. Patients consisted of men and women of varying body size (lean to severely obese). Samples were collected at the time of operation in the fasting state. Samples consisted of subcutaneous and omental adipose tissue as well as a blood sample from lean and obese men and women removed in the fasting state at the time of surgery.

2010-06-20 | E-GEOD-15524 | biostudies-arrayexpress

Whole tissue mRNA expression from severely obese individuals: subcutaneous and visceral adipose tissues

Project description:Subcutaneous adipose tissue and visceral adipose tissue samples were obtained from severely obese individuals that underwent bariatric surgery. The goal of this study was to compare genome-wide gene expression levels in the two tissue types from healthy and unhealthy severely obese individuals. Whole-transcriptome subcutaneous adipose tissue gene expression levels were determined in 73 individuals with a BMI >35 kg/m2. Whole-transcriptome visceral adipose tissue gene expression levels were determined in 69 individuals with a BMI >35 kg/m2. Modules of co-expressed genes likely to be functionally related were identfied and correlated with BMI, plasma levels of glucose, insulin, HbA1c, triglycerides, non-esterified fatty acids, ALAT, ASAT, C-reactive protein, and LDL- and HDL cholesterol.

2010-06-03 | GSE22070 | GEO

Microarray analysis of subcutaneous adipose tissue obtained in 27 moderate obese women

Project description:The mechanisms responsible for weight loss-induced improvement in insulin sensitivity are partially understood. Greater insight can now be achieved through deep phenotyping and data integration. Here, we used an integrative approach to investigate associations between changes in insulin sensitivity and variations in lifestyle factors (diet and physical activity), subcutaneous adipose tissue (sAT) gene expression, metabolomics in serum, urine and feces, and gut microbiota composition after a 6-week calorie restriction period in overweight and obese adults

2018-03-27 | GSE112307 | GEO

Microarray of isolated adipocytes from subcutaneous adipose tissue of severely obese patients compared to lean controls

Project description:The aim of the project was to compare global gene expression in adipocytes from obese patients and lean controls. Subcutaneous adipose tissue was collected from severely obese patients undergoing bariatric surgery (average body-mass index (BMI) of 45.5 kg/m2 (n = 12, thereof 4 men) and healthy lean patients undergoing hernia repairs (average BMI of 24.2 kg/m2 (n = 12, thereof 7 men), between 27 and 56 years of age. Adipocytes were isolated by collagenase treatment of adipose tissue, followed by filtering and centrifugation. Floating adipocytes were lysed in Qiazol before RNA purification and microarray analysis.

2018-12-12 | E-MTAB-6728 | biostudies-arrayexpress

Expression profiling of human tissues from obese patients

Project description:This experiment captures the expression profiling in obese with type 2 diabetes and non-diabetic patients, in relevant tissues from the disease: liver, subcutaneous and visceral adipose tissues, and whole blood. Samples were obtained during bariatric surgery and preserved in RNA later at -70 C until the nucleic acid extraction.

2018-02-15 | E-MTAB-5288 | biostudies-arrayexpress

DNA Methylation analysis of human tissues from obese patients

Project description:This experiment captures the DNA methylation in obese patients with type 2 diabetes in relevant tissues from the disease: liver, subcutaneous and visceral adipose tissues, and whole blood. Samples were obtained during bariatric surgery and preserved in RNAlater at -70 C in RNAlater, until the nucleic acid extraction.

2018-02-15 | E-MTAB-5291 | biostudies-arrayexpress

Expression data from Adipose Stem Cells (ASC) from morbidly obese and non-obese individuals

Project description:The adipose tissue is an endocrine regulator and a risk factor for atherosclerosis and cardiovascular disease when by excessive accumulation induces obesity. Although the adipose tissue is also a reservoir for stem cells (ASC) their function and “stemcellness” has been questioned. Our aim was to investigate the mechanisms by which obesity affects subcutaneous white adipose tissue (WAT) stem cells. We used microarrays to analyze differences in transcriptomic profiles between the adipose stem cells from morbidly obese and non-obese individuals. Subcutaneous white adipose tissues that were obtained during bariatric surgery (Obese) or liposuction (Lean) were donated by patients after obtaining informed consent. Three cases with BMI>40 kg/m2 (ASCmo) and three controls with BMI<25 kg/m2 (ASCn) were selected and processed extracting total RNA, processing and hybridizating on microarrays.

2013-09-17 | E-GEOD-48964 | biostudies-arrayexpress

Individualized analysis of subcutaneous and omental abdominal adipose tissue from non-obese and obese subjects

2009-04-03 | GSE15524 | GEO

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