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The transcriptional repressor Polycomb group factor 6 (PCGF6) negatively regulates dendritic cell activation and promotes quiescence

ABSTRACT: We identified that DC activation and immunogenicity are regulated by the transcriptional repressor polycomb group factor 6 (PCGF6). Pcgf6 is rapidly downregulated upon stimulation and this downregulation is necessary to permit full DC activation. Silencing PCGF6 expression enhanced both spontaneous and stimulated DC activation. We show that PCGF6 associates with the H3K4me3 demethylase JARID1c, and together they negatively regulate H3K4me3 levels in DCs. Our results identify two key regulators – PCGF6 and JARID1c – that temper DC activation, and implicate active transcriptional silencing via histone demethylation as a previously unappreciated mechanism for regulating DC activation and quiescence

ORGANISM(S): Mus musculus

PROVIDER: GSE83640 | GEO | 2016/07/13

SECONDARY ACCESSION(S): PRJNA326590

REPOSITORIES: GEO

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Polycomb Group Protein Pcgf6 Acts as a Master Regulator to Maintain Embryonic Stem Cell Identity

Project description:We report that the PRC1 component polycomb group ring finger 6 (Pcgf6) is required to maintain embryonic stem cell (ESC) identity. In contrast to canonical PRC1, Pcgf6 acts as a positive regulator of transcription and binds predominantly to promoters bearing active chromatin marks.Â Pcgf6Â is expressed at high levels in ESCs, and knockdown reduces the expression of the core ESC regulatorsÂ Oct4,Sox2, andÂ Nanog. Conversely,Â Pcgf6Â overexpression prevents downregulation of these factors and impairs differentiation. In addition, Pcgf6 enhanced reprogramming in both mouse and human somatic cells. The genomic binding profile of Pcgf6 is highly similar to that of trithorax group proteins, but not of PRC1 or PRC2 complexes, suggesting that Pcgf6 functions atypically in ESCs. Our data reveal novel roles for Pcgf6 in directly regulatingÂ Oct4,Â Nanog,Â Sox2, andÂ Lin28 expression to maintain ESC identity. To identify Pcgf6-bound genomic DNA regions in mouse embryonic stem cells, we fixed mouse ESCs and isolated Pcgf6-bound genomic DNA regions for deep sequencing analysis.

2016-07-28 | E-GEOD-84905 | biostudies-arrayexpress

PCGF6, a polycomb group protein, regulates mesodermal lineage differentiation in murine ES cells and functions in iPS reprogramming

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RNA-seq for the tissues of Pcgf6 knockout mice and wild type mice

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Polycomb Group Protein Pcgf6 Acts as a Master Regulator to Maintain Embryonic Stem Cell Identity

Project description:We report that the PRC1 component polycomb group ring finger 6 (Pcgf6) is required to maintain embryonic stem cell (ESC) identity. In contrast to canonical PRC1, Pcgf6 acts as a positive regulator of transcription and binds predominantly to promoters bearing active chromatin marks. Pcgf6 is expressed at high levels in ESCs, and knockdown reduces the expression of the core ESC regulators Oct4,Sox2, and Nanog. Conversely, Pcgf6 overexpression prevents downregulation of these factors and impairs differentiation. In addition, Pcgf6 enhanced reprogramming in both mouse and human somatic cells. The genomic binding profile of Pcgf6 is highly similar to that of trithorax group proteins, but not of PRC1 or PRC2 complexes, suggesting that Pcgf6 functions atypically in ESCs. Our data reveal novel roles for Pcgf6 in directly regulating Oct4, Nanog, Sox2, and Lin28 expression to maintain ESC identity.

2016-07-28 | GSE84905 | GEO

Expression regulation by Pcgf6 in mouse embryonic stem cells

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2017-01-14 | GSE92476 | GEO

Pcgf6 suppresses Myc-induced lymphomagenesis

Project description:Pcgf6 suppresses Myc-induced lymphomagenesis

| PRJNA785557 | ENA

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2023-12-18 | GSE228479 | GEO

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