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Effect of Metformin and AICAR treatments in the prostate cancer cell line LNCaP

ABSTRACT: Transcriptional profiling of human prostate cancer cell line LNCaP treated with Metformin or AICAR compared to control non-stimulated LNCaP.

ORGANISM(S): Homo sapiens

PROVIDER: GSE83673 | GEO | 2017/08/11

SECONDARY ACCESSION(S): PRJNA326678

REPOSITORIES: GEO

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Metformin regulated genes in human prostate cancer cells

Project description:The biguanide metformin has been shown to not only reduce circulating glucose levels but also suppress in vitro and in vivo growth of prostate cancer. However, the mechanisms underlying the anti-tumor effects of metformin in advanced prostate cancers are not fully understood. The goal of the present study was to define the signaling pathways regulated by metformin in androgen-receptor (AR) positive, castration-resistant prostate cancers. Our group used RNA sequencing (RNA-seq) to examine genes regulated by metformin within the C4-2 human prostate cancer cell line. Western blot analysis and quantitative RT-PCR were used to confirm alterations in gene expression and further explore regulation of protein expression by metformin. Data from the RNA-seq analysis revealed that metformin alters the expression of genes products involved in metabolic pathways, the spliceosome, RNA transport, and protein processing within the endoplasmic reticulum. Gene products involved in ErbB, insulin, mTOR, TGF-, MAPK, and Wnt signaling pathways are also regulated by metformin. A subset of metformin-regulated gene products were genes known to be direct transcriptional targets of p53 or AR. Together, our results suggest metformin regulates multiple pathways linked to tumor growth and progression within advanced prostate cancer cells.

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Expression data from normal Prostate epithelial cells (PrEC) and the LNCaP cancer cell line

Project description:To identify genomic regions which display concordant gene expression in prostate cancer, we performed expression profiling of normal prostate epithelial cells (PrEC) and the prostate cancer cell line LNCaP. These expression arrays were integrated ChIP-on-chip studies of active and repressive epigenetic marks in same cells to discover and characterise regions of Long Range Epigenetic Silencing (LRES) in prostate cancer.

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lncRNA-PCAT1 knockdown effect on the gene expression of androgen independent LNCaP (LNCaP-AI) cell line

Project description:We generated and characterized an androgen-independent LNCaP-AI cell line by long-term culture of androgen-dependent LNCaP cells in RPMI-1640 medium containing charcoal-stripped serum. This approach used to generate the line mimics the castration resistant condition for treating prostate cancer, supporting the relevance of the LNCAP-AI cell line to Castration Resistant Prostate Cancer.

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Expression profile of LNCaP prostate cancer cell line treated with DMSO or BP

Project description:N-butylidenephthalide (BP) exhibits its antitumor effect in a variety of cancer cell lines. However, little is known about its effects on prostate cancer. The objective of this study was to obtain additional insights into the mechanisms involved in BP induced cell death in human prostate cancer cells. To determine the mechanisms of BP-induced growth arrest and cell death in prostate cancer cell lines, we performed a microarray study to identify alterations in gene expression induced by treatment with BP in the LNCaP cells. LNCaP cellss were selected to treated with BP for 3 or 24hr for RNA extraction and hybridization on Affymetrix microarrays.

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Comparison of the prostate cancer cell line LNCaP and its androgen insensitive derivative C4-2B

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2007-08-07 | E-GEOD-7702 | biostudies-arrayexpress

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