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MiRNAs regulate dynamic transcriptional changes of broiler embryos in response to modification of the incubation temperature (Musculus gastrocnemius)

ABSTRACT: miRNA expression was analysed of samples derived from M. gastrognemius of day 10 and day 13 chicken embryos and day 35 broiler after variation of incubation temperature

ORGANISM(S): synthetic construct Gallus gallus

PROVIDER: GSE83704 | GEO | 2017/05/31

SECONDARY ACCESSION(S): PRJNA326808

REPOSITORIES: GEO

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MiRNAs regulate dynamic transcriptional changes of broiler embryos in response to modification of the incubation temperature (Musculus pectoralis)

Project description:miRNA expression was analysed of samples derived from M. pectoralis of day 10 and day 13 chicken embryos and day 35 broiler after variation of incubation temperature

2017-05-31 | GSE83703 | GEO

Pre- and Post-hatch transcriptomic response of chicken Musculus gastrocnemius to transient variation of incubation temperature

Project description:After elevated and reduced incubation temperature during embryonic days (ED) 7-10 and 10-13 changes of gene expression were determined at ED 10, ED 13, and post-hatch at day (D) 35

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Effect of an immune challenge and feed supplements on broiler chicken individual breast muscle protein synthesis

Project description:Optimization of broiler chicken breast muscle protein accretion is key for the efficient production of poultry meat, whose demand is steadily increasing. In a context where antimicrobial growth promoters use is being restricted, it is important to find alternatives as well as to characterize the effect of immunological stress on broiler chicken growth. Despite of its importance, research on broiler chicken muscle protein dynamics has been mostly limited to the study of mixed protein turnover. The present study aims to characterize the effect of a bacterial challenge and the feed supplementation of a citrus and a cucumber extract on broiler chicken individual breast muscle proteins fractional synthesis rates (FSR) using a recently developed dynamic proteomics pipeline. 21 day-old broiler chickens were administered a single 2H2O dose before being culled at different timepoints. A total of 60 breast muscle protein extracts from five experimental groups (Unchallenged, Challenged, Control Diet, Diet 1 and Diet 2) were analyzed using a DDA proteomics approach. Proteomics data was filtered in order to reliably calculate multiple proteins FSR making use of a newly developed bioinformatics pipeline. Broiler breast muscle proteins FSR uniformly decreased following a bacterial challenge, this change was judged significant for 15 individual proteins, the two major functional clusters identified as well as for mixed breast muscle protein. Citrus or cucumber extract feed supplementation did not show any effect on the breast muscle protein FSR of immunologically challenged broilers. The present study has identified potential predictive markers of breast muscle growth and provided new information on broiler chicken breast muscle protein turnover which could be essential for improving the efficiency of broiler chicken meat production.

2024-04-04 | PXD044325 | Pride

Fold change of expression in Aseel (an indigenous chicken breed) as compared to broiler chicken in 7th day of embryonic stage

Project description:The expression of genes were analysed in 7th day of embryonic stage between Aseel, an indigenous slow-growing chicken, and control broiler, a fast-growing broiler chicken line. The whole embryo was collected in TRIZOL and total RNA was isolated. The expression profile of gene was determined in 64k Agilent chicken microarray chip. The Cy3 dye was used for detection. The fold change of expression was analysed in Aseel as compared to broiler chicken line.

2014-10-17 | GSE62443 | GEO

Fold change of expression in Aseel (an indigenous chicken breed) as compared to broiler chicken in muscle of 18th day of embryonic stage

Project description:The expression of genes were analysed in muscle of 18th day of embryonic stage between Aseel, an indigenous slow-growing chicken, and control broiler, a fast-growing broiler chicken line. The whole embryo was collected in TRIZOL and total RNA was isolated. The expression profile of gene was determined in 64k Agilent chicken microarray chip. The Cy3 dye was used for detection. The fold change of expression was analysed in Aseel as compared to broiler chicken line.

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Pre- and Post-hatch transcriptomic response of chicken Musculus pectoralis major to transient variation of incubation temperature

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MiRNAs regulate dynamic transcriptional changes of broiler embryos in response to modification of the incubation temperature

Project description:MiRNAs regulate dynamic transcriptional changes of broiler embryos in response to modification of the incubation temperature

| PRJNA326803 | ENA

miRNAs regulate acute transcriptional changes in broiler embryos in response to modification of incubation temperature.

Project description: Not available

| S-EPMC6063901 | biostudies-literature

F8 broiler by Leghorn and broiler by Fayoumi spleen at day 7 and 8 after challenge with SE

Project description:In the two F8 advanced crosses of broiler by Leghorn and broiler by Fayoumi, birds at day 1 were challenged with Salmonella enteritidis (SE). Spleen were collected at day 7 and 8. SE bacterial load in spleen were measured. Based on the bacterial load, birds were divided into high and low SE load groups. Keywords: Salmonella enteritidis challenge

2006-06-01 | GSE4273 | GEO

The dynamic methylome of the broiler chicken as a resource for livestock performance biomarker development

Project description:The broiler chicken is the globally most important source of commercially produced meat. While genetic approaches have played an important role in the development of chicken stocks, little is known about chicken epigenetics. We have now systematically analyzed the chicken DNA methylation toolkit and DNA methylation landscape. While overall DNA methylation patterns were similar to mammals, sperm DNA appeared distinctly hypomethylated, which correlates with the absence of the DNMT3L cofactor in the chicken genome. Additional analysis also revealed the presence of low-methylated regions in the chicken methylome, which are conserved gene regulatory elements that show tissue-specific methylation patterns. We also used whole-genome bisulfite sequencing to establish 56 single-base resolution methylomes of the broiler chicken from various tissues and developmental time points. Data analysis confirmed tissue-specific and time-dependent methylation patterns that were used to establish a DNA methylation clock to predict sample age and measure stock performance. Our study thus provides a comprehensive resource for the chicken methylome and establishes a novel epigenetic tool for livestock performance analysis.

2020-11-30 | GSE146620 | GEO

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