Dataset Information


Expression data from hMSC-TERT4 cells after siZNF25 knockdown

ABSTRACT: Differentiation of human skeletal stem cells (hMSC) into osteoblasts is regulated by a few well described transcription factors. Our study used clustering and gene expression data to identify a novel transcription factor. ZNF25, which we showed is involved in osteoblast differentiation. We used microarrays to study gene expression of hMSC-TERT4 cells after siZNF25 knockdown. hMSC-TERT4 cells were sampled as undifferentiated hMSC and as differentiated osteoblasts. Overall design: hMSC-TERT4 cells were transfected with siZNF25. Gene expression was analysed using Affymetrix microarrays at days 0 post osteoblast induction and days 14 post osteoblast induction.

INSTRUMENT(S): [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Natalie Twine 

PROVIDER: GSE83901 | GEO | 2016-09-01



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Transcription factor ZNF25 is associated with osteoblast differentiation of human skeletal stem cells.

Twine Natalie A NA   Harkness Linda L   Kassem Moustapha M   Wilkins Marc R MR  

BMC genomics 20161104 1

The differentiation of human bone marrow derived skeletal stem cells (known as human bone marrow stromal or mesenchymal stem cells, hMSCs) into osteoblasts involves the activation of a small number of well-described transcription factors. To identify additional osteoblastic transcription factors, we studied gene expression of hMSCs during ex vivo osteoblast differentiation.Clustering of gene expression, and literature investigation, revealed three transcription factors of interest - ZNF25, ZNF60  ...[more]

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