Genomics

Dataset Information

38

Gene expression profile in the small Maf-deficient livers.


ABSTRACT: To elucidate the functional roles of sMafs in the adult liver, we conditionally targeted the sMaf genes using a transgenic complementation rescue approach. MafF-/-::MafG-/-::MafK-/- (F0G0K0) mice are embryonic lethal but can be rescued by complementation of transgenic MafG expression under the regulation of the MafG regulatory domain (MGRD). Therefore, we rescued F0G0K0 mice using a MGRD transgenic mouse line with a MafG gene flanked with loxP (fMafG) sequences so that the MafG gene could be deleted by Cre-mediated recombination. The Albumin(Alb)-Cre transgenic mice were used to delete fMafG gene specifically in the liver. The genotype used are MafF-/-::MafG-/-::MafK-/-::MGRD-fMafG::Alb-Cre (liver-specific sMaf CKO) and MafF-/-::MafG+/-::MafK-/-::MGRD-fMafG::Alb-Cre (control). Overall design: The microarray analyses were performed using three independent RNA samples from livers of liver-specific sMaf CKO and control mice at 5 weeks of age.

INSTRUMENT(S): Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)

SUBMITTER: Masayuki Yamamoto  

PROVIDER: GSE84001 | GEO | 2017-05-01

SECONDARY ACCESSION(S): PRJNA327793

REPOSITORIES: GEO

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Publications

Small Maf deficiency recapitulates the liver phenotypes of Nrf1- and Nrf2-deficient mice.

Katsuoka Fumiki F   Yamazaki Hiromi H   Yamamoto Masayuki M  

Genes to cells : devoted to molecular & cellular mechanisms 20161010 12


Nrf1 and Nrf2 (NF-E2-related factors 1 and 2, respectively) are transcription factors that belong to the Cap'n'collar (CNC) family and play critical roles in various tissues, including the liver. Liver-specific Nrf1 knockout mice show hepatic steatosis, accompanied by dysregulation of various metabolic genes. Nrf2 knockout mice show impairment in the induction of antioxidant and xenobiotic-metabolizing enzyme genes. Although it has been shown that small Maf (sMaf) proteins act as obligatory part  ...[more]

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