Transcriptomics,Genomics

Dataset Information

95

Reprogrammed neurons from adult dermal fibroblasts of patients with Huntington's disease


ABSTRACT: Reprogrammed neurons from adult dermal fibroblasts of patients with Huntington's disease Overall design: De-identified human adult dermal fibroblasts from patients with Huntington's disease or age- and gender-matched healthy controls were obtained from the Coriell Institute for Medical Research Biorepository. These fibroblast samples were transduced with lentivirus to express the brain enriched microRNAs, miR-9/9* and miR-124 and striatal-enriched transcription factors BCL11B (Also known as CTIP2), DLX1, DLX2, and MYT1L to directly convert fibroblasts into striatal neurons as previously established (Victor et al., Neuron 2014; Richner et al., Nat. Prot. 2015). Total mRNA from reprogrammed neurons and non-converted fibroblasts was collected and prepared for RNA-seq analysis 32 days after viral transduction.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

SUBMITTER: Bo Zhang  

PROVIDER: GSE84013 | GEO | 2017-12-11

SECONDARY ACCESSION(S): PRJNA327817

REPOSITORIES: GEO

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