Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Localization of Snf5 in wild-type and knockout yeast

ABSTRACT: ChIP-chip assays to determine the localization of Snf5 in wild-type and knockout yeast.

ORGANISM(S): Saccharomyces cerevisiae S288C Saccharomyces cerevisiae

PROVIDER: GSE84065 | GEO | 2017/01/16

SECONDARY ACCESSION(S): PRJNA327924

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Localization of TAP-proteins in wild-type and tagging yeast

Project description:ChIP-chip assays to determine the localization of TAP-protein in wild-type and tagging yeast.

2017-01-16 | GSE84066 | GEO

Localization of wildtype Ioc4 in wildtype and set2 yeast strains. Localization of Ioc4 mutants (dPWWP, 2KE, dINS) in wildtype yeast strains.

Project description:ChIP-chip assays to assess the localization of FLAG-tagged, wildtype and mutant Ioc4 in yeast.

2022-01-24 | GSE165984 | GEO

Localization of Isw1-3xFLAG in wild-type yeast.

Project description:ChIP-chip assays to determine the localisation of 3xFLAG tagged Isw1 in wild-type yeast.

2011-09-07 | GSE31301 | GEO

Localization of Isw1-3xFLAG in wild-type yeast.

Project description:ChIP-chip assays to determine the localisation of 3xFLAG tagged Isw1 in wild-type yeast. Two color experiment. ChIP/Input. Biological replicates=3. Other data used in this study are provided in GEO Series GSE31015 and GSE31833.

2011-09-06 | E-GEOD-31301 | biostudies-arrayexpress

Chromosome localization of cohesin oligomers in mid-M arrested yeast cells

Project description:We report the genomic localization of cohesin oligomers in nocodazole arrested yeast cells. Two alleles of SMC3 were expressed in yeast cells, one fused to BirA enzyme and the other tagged with AviTag. Cohesin oligomers were biotinylated and ChIP with streptavidin beads. As control experiments, cohesin localization on chromosome was determined in strains expresses freely diffusable BirA enzyme, where all Smc3 proteins were biotinylated; non-specific ChIP were determined in strains with no BirA.

2020-08-31 | GSE157155 | GEO

Loss of Snf5 and the formation of an aberrant SWI/SNF complex

Project description:Aberrant forms of the SWI/SNF chromatin remodeling complex are associated with human disease. Loss of the Snf5 subunit of SWI/SNF is a driver mutation in pediatric rhabdoid cancers and forms aberrant sub-complexes that are not well characterized. We determined the effects of loss of Snf5 on the composition, nucleosome binding, recruitment and remodeling activities of yeast SWI/SNF. The Snf5 subunit interacts with the ATPase domain of Snf2 and forms a submodule consisting of Snf5, Swp82 and Taf14 as shown by mapping SWI/SNF subunit interactions by crosslinking-mass spectrometry and subunit deletion followed by immunoaffinity chromatography. Snf5 promoted binding of the Snf2 ATPase domain to nucleosomal DNA, enhanced its catalytic activity and facilitated nucleosome remodeling. Snf5 was required for acidic transcription factors to recruit SWI/SNF to chromatin. RNA-seq analysis suggested that both the recruitment and catalytic functions mediated by Snf5 are required for SWI/SNF regulation of gene expression.

2017-01-30 | GSE85460 | GEO

Histone H3 K36 trimethylation in wild-type BY4741 yeast strains

Project description:ChIP-on chip assays to measure the change in histone H3 K36 trimethylation over the yeast genome in wild-type yeast strains.

2012-07-25 | GSE37105 | GEO

Genome-wide maps of chromatin remodeler SNF5 in human pluripotent cells.

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of SNF5 binding in human pluripotent embryonic carcinoma NCCIT and SNF5 overexpressed NCCIT cells. We generated genome-wide cSNF5 maps of NCCIT and SNF5 overexpressed NCCIT cells from chromatin immunoprecipitated DNA. SNF5 and OCT4 seem not to share their binding in OCT4 centered binding plot in control, while SNF5 overexpression directs SNF5 to OCT4 target genes.

2013-01-01 | GSE36134 | GEO

Elevated levels of H3K27me3 in Snf5-deficient cells

Project description:Epigenetic alterations have been increasingly implicated in oncogenesis. Analysis of Drosophila mutants suggests that Polycomb and SWI/SNF complexes can serve antagonistic developmental roles. However, the relevance of this relationship to human disease is unclear. Here we have investigated functional relationships between these epigenetic regulators in oncogenic transformation. Mechanistically, we show that loss of the SNF5 tumor suppressor leads to elevated expression of the Polycomb gene EZH2 and that Polycomb targets are broadly H3K27-trimethylated and repressed in SNF5-deficient fibroblasts and cancers. Further, we show antagonism between SNF5 and EZH2 in the regulation of stem cell-associated programs and that Snf5 loss activates those programs. Finally, using conditional mouse models, we show that inactivation of Ezh2 blocks tumor formation driven by Snf5 loss.

2010-10-19 | GSE23658 | GEO

Genome-wide maps of chromatin remodeler SNF5 in human pluripotent cells.

2013-01-01 | E-GEOD-36134 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data