Transcriptome analysis of adenine-treated murine microglial cells
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ABSTRACT: In previous study, we demonstrated adenine-induced cell signaling on anti-inflammation in BV2 cells. To investigate the changes of transcriptome mediated by adenine, we used microarrays to compare the difference in expression levels.
Project description:In previous study, we demonstrated that adenine could activate AMPK signaling in 3T3 fibroblast. To investigate the changes of transcriptome in differential 3T3 cells by adenine stimulation, we used microarrays to analyze undifferentited annd differentiated 3T3-L1 adipocytes in mRNA levels.
Project description:Previous experiments have shown that E. feacalis increases EHEC virulence by secreting adenine, this RNAseq aims to understand the molecular mechanism underlaying adenine role on EHEC
Project description:Investigation of transcriptome changes in four human cell lines (BJ, BJ-5ta, U2OS and HeLa) after treatment for 24 hours with nicotinamide adenine dinucleotide (NAD+). Cells were untreated as the control condition. Nanopore sequencing of cDNA was performed after library preparation with the ONT SQK-PCB109 kit.