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Analysis of gene expression in TET1 depleted colorectal cancer HCT116 cells

ABSTRACT: We aimed to analyze the relationship between TET1 and aberrant CpG methylation in colorectal cancer (CRC). We established two stable TET1 knockdown clones and negative control clones of HCT116 cells, and carried out gene expression analysis with Agilent Human Gene Expression microarray kit.

ORGANISM(S): Homo sapiens

PROVIDER: GSE84398 | GEO | 2017/01/01

SECONDARY ACCESSION(S): PRJNA329085

REPOSITORIES: GEO

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Analysis of DNA methylation in TET1 depleted colorectal cancer HCT116 cells

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Analysis of gene expression in TET1 depleted colorectal cancer Colo320DM cells

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Transcription changes due to TET1 knockdown in HCT116 colorectal cancer cells.

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Transcription profiling of human colorectal cell line HCT116 DICER Exon5 knockout cells

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Expression signature in a human colorectal cancer cell line HCT116 transfected with miR-1 precursor molecule

Project description:We identified that miR-1 is silenced in association with CpG island hypermethylation in a colorectal cancer (CRC) cell line, HCT116. To determine whether miR-1 serves as a tumor suppressor in CRC, we transfected CRC cell lines with a miR-1 precursor molecule or a negative control, and carried then out a series of MTT assays. Forty-eight hours after transfection, we observed that ectopic expression of miR-1 moderately suppressed growth in all three cell lines. To further clarify the effect of the miRNA, we next performed gene expression microarray analysis in HCT116 cells transfected with a miR-1 precursor molecule or a negative control. We found that 2769 probe sets were downregulated (> 1.5-fold) by ectopic miR-1 expression, and gene ontology analysis revealed that “extracellular regions”, “membrane” and “response to wound healing” genes were significantly enriched among the downregulated genes. HCT116 cells were transfected with a Pre-miR-1 miRNA Precursor Molecule (Ambion) or Pre-miR miRNA Molecules Negative Control #1 (Ambion). Forty-eight hours after transfection, total RNA extraction were carried out, and gene expression signatures were analyzed.

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microRNA expression signatures in colorectal cancer cell lines with demethylating treatment

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