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Expression microarray analysis of RHOXF1 and RHOXF2 transfected HEK293 cells

ABSTRACT: The X-linked reproductive homeobox (RHOX) gene cluster encodes transcription factors displaying preferential expression in reproductive tissues. They have important roles in human male fertility based on phenotypic defects of Rhox-mutant mice and the finding that aberrant RHOX promoter methylation is strongly associated with abnormal human sperm parameters. The aim of this study was to analyze the molecular mechanism of RHOX action in humans. Using genome-wide analysis, we investigated the identity of genes regulated by the known human RHOX transcription factors: RHOXF1 and RHOXF2/2B. HEK293 cells were transiently transfected with either RHOX expression (RHOXF1; RHOXF2) or an empty control vector. Afterwards, differently expressed genes were identified by microarray analysis after 24h and 48h of transfection. Thereby we could identify genes that are differently regulated in response to RHOX overexpression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE84463 | GEO | 2016/07/16

SECONDARY ACCESSION(S): PRJNA329254

REPOSITORIES: GEO

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Expression microarray analysis of RHOXF1 and RHOXF2 transfected HEK293 cells

Project description:The X-linked reproductive homeobox (RHOX) gene cluster encodes transcription factors displaying preferential expression in reproductive tissues. They have important roles in human male fertility based on phenotypic defects of Rhox-mutant mice and the finding that aberrant RHOX promoter methylation is strongly associated with abnormal human sperm parameters. The aim of this study was to analyze the molecular mechanism of RHOX action in humans. Using genome-wide analysis, we investigated the identity of genes regulated by the known human RHOX transcription factors: RHOXF1 and RHOXF2/2B. HEK293 cells were transiently transfected with either RHOX expression (RHOXF1; RHOXF2) or an empty control vector. Afterwards, differently expressed genes were identified by microarray analysis after 24h and 48h of transfection. Thereby we could identify genes that are differently regulated in response to RHOX overexpression. Total RNA was isolated from RHOX (RHOXF1; RHOXF2) and control transfected HEK293 cells after 24h and 48h of transfection. Affymetrix GeneChip Human Exon 1.0 ST arrays were used to evaluate changes in mRNA expression in RHOX transfected samples compared to control transfected samples (analyzed in biological triplicates). Data were processed and analyzed using Affymetrix Gene Expression Console and Affymetrix transcriptome analysis console.

2016-07-16 | E-GEOD-84463 | biostudies-arrayexpress

Transcription profiling of mouse 15P-1 Sertoli cell clones stably transfected with mouse Rhox5-expression vector reveals androgen-Induced Rhox homeobox genes modulate the expression of AR-regulated genes

Project description:Rhox5, the founding member of the reproductive homeobox on the X chromosome (Rhox) gene cluster, encodes a homeodomain-containing transcription factor that is selectively expressed in Sertoli cells, where it promotes the survival of male germ cells. To identify Rhox5-regulated genes, we generated 15P-1 Sertoli cell clones expressing physiological levels of Rhox5 from a stably transfected expression vector. Microarray analysis identified many genes altered in expression in response to Rhox5, including those encoding proteins controlling cell cycle regulation, apoptosis, metabolism, and cell-cell interactions. Fifteen of these Rhox5-regulated genes were chosen for further analysis. Analysis of Rhox5-null male mice indicated that at least 9 of these are Rhox5-regulated in the testes in vivo. Many of them have distinct postnatal expression patterns and are regulated by Rhox5 at different postnatal time points. Most of them are expressed in Sertoli cells, indicating that they are candidates to be directly regulated by Rhox5. Transfection analysis with expression vectors encoding different mouse and human Rhox family members revealed that the regulatory response of a subset of these Rhox5-regulated genes is both conserved and redundant. Given that Rhox5 depends on AR for expression in Sertoli cells, we examined whether some Rhox5-regulated genes are also regulated by androgen receptor (AR). We provide several lines of evidence that this is the case, leading us to propose that RHOX5 serves as a key intermediate transcription factor that directs some of the actions of AR in the testes. Experiment Overall Design: Four 15P-1 Sertoli cell clones stably transfected with mouse Rhox5-expression vector were used for microarray analysis. Among them, two clones (6 and 24) expressed highest level of Rhox5 mRNA, and other two clones (14 and 16) expressed the lowest level of Rhox5 mRNA.

2009-11-10 | E-GEOD-18785 | biostudies-arrayexpress

The Rhox gene cluster suppresses germline LINE1 transposition

Project description:Transposable elements (TEs) are mobile sequences that engender widespread mutations and thus are a major hazard that must be silenced. The most abundant active class of TEs in mammalian genomes is long interspersed element class 1 (LINE1). Here, we report that LINE1 transposition is suppressed in the male germline by transcription factors encoded by a rapidly evolving X-linked homeobox gene cluster. LINE1 transposition is repressed by many members of this RHOX transcription factor family, including those with different patterns of expression during spermatogenesis. One family member—RHOX10—suppresses LINE1 transposition during fetal development in vivo when the germline would otherwise be susceptible to LINE1 activation because of epigenetic reprogramming. We provide evidence that RHOX10 suppresses LINE transposition by inducing Piwil2, which encodes a key component in the Piwi-interacting (pi) RNA pathway that protects against TEs. The ability of RHOX transcription factors to suppress LINE1 is conserved in humans, but is lost in RHOXF2 mutants from several infertile human patients, raising the possibility that loss of RHOXF2 causes human infertility by allowing uncontrolled LINE1 expression in the germline. Together, our results support a model in which the Rhox gene cluster is in an evolutionary arms race with TEs, resulting in expansion of the Rhox gene cluster to suppress TEs in different biological contexts.

2021-05-20 | GSE160600 | GEO

Extracellular ATP causes ROCK I-dependent bleb formation in P2X7-transfected HEK293 cells.

Project description: Not available

| S-EPMC165666 | biostudies-literature

Androgen-Induced Rhox Homeobox Genes Modulate the Expression of AR-Regulated Genes

2009-10-29 | GSE18785 | GEO

mRNA profiling after Inhibition of the lncRNA Ntep

Project description:To investigate the effect of inhibition of Ntep on 3T3 cells, we treated cells for 48h with GapmeR control or Gapmer against Ntep Afterwards total RNA was isolated and used for microarray profiling.

2017-08-08 | GSE94049 | GEO

Mus musculus

Project description:Androgen-Induced Rhox Homeobox Genes Modulate the Expression of AR-Regulated Genes

| PRJNA121257 | ENA

Calcium Imaging of GPCR Activation Using Arrays of Reverse Transfected HEK293 Cells in a Microfluidic System.

Project description: Not available

| S-EPMC5855233 | biostudies-literature

Homo sapiens

Project description:Mutation analysis of human RHOX genes

| PRJNA329175 | ENA

Transcription profiling of human HEK293 cells transfected with EWS-FLI1 (Ewing sarcoma family fusion gene)

Project description:Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. DNA-microarray analysis was performed using Affymetrix HG-U133A microarrays.(see Staege et al. Cancer Res. 2004)

2007-08-25 | E-GEOD-1822 | biostudies-arrayexpress

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