Dataset Information


Effect of benzimidazole derivative C162 on Staphylococcus aureus virulence

ABSTRACT: From a Caenorhabditis elegans - S. aureus anti-infective screen, we identified benzimidazole derivative C162 as one of the potential anti-infective candidate that rescued the infected-nematodes from infection. This compound was found to exhibit anti-biofilm activity against S. aureus. To investigate the transcriptome profile of S. aureus in response to benzimidazole derivative C162, a genome-wide transcriptome analysis was performed on C162-treated S. aureus using the Affymetrix GeneChip S. aureus Genome Arrays. Our main interest is to look at the expression profiles of the biofilm-associated and virulence genes. Overall design: We conducted three independent microarray experiments (biological replicates) in the absence (control) and the presence (treated) of 6.25 µM benzimidazole derivative C162. We calculated the fold change as the ratio between the signal averages of three untreated and three C162-treated cultures at a single time-point (overnight incubation for 16-18 hours).

INSTRUMENT(S): [S_aureus] Affymetrix S. aureus Genome Array


PROVIDER: GSE84485 | GEO | 2018-02-13


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Suppression of Staphylococcus aureus biofilm formation and virulence by a benzimidazole derivative, UM-C162.

Kong Cin C   Chee Chin-Fei CF   Richter Katharina K   Thomas Nicky N   Abd Rahman Noorsaadah N   Nathan Sheila S  

Scientific reports 20180209 1

Staphylococcus aureus is a major cause of nosocomial infections and secretes a diverse spectrum of virulence determinants as well as forms biofilm. The emergence of antibiotic-resistant S. aureus highlights the need for alternative forms of therapeutics other than conventional antibiotics. One route to meet this need is screening small molecule derivatives for potential anti-infective activity. Using a previously optimized C. elegans - S. aureus small molecule screen, we identified a benzimidazo  ...[more]

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