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Ex vivo sorted murine pro-B cells: Wild-type versus Pax5-/-

ABSTRACT: Transcriptional profiling of mouse pro-B cells comparing wild-type and Pax5-deficient cells Keywords: Genetic modification

ORGANISM(S): Mus musculus

PROVIDER: GSE8458 | GEO | 2007/07/13

SECONDARY ACCESSION(S): PRJNA105351

REPOSITORIES: GEO

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In vitro cultured murine pro-B cells: Wild-type versus Pax5-/-

Project description:Transcriptional profiling of mouse pro-B cells comparing wild-type and Pax5-deficient cells Keywords: Genetic modification

2007-07-13 | GSE8457 | GEO

In vitro cultured and Ex vivo sorted murine pro-B cells: Wild-type versus Pax5-/-

Project description:This SuperSeries is composed of the SubSeries listed below.

2007-07-13 | GSE8461 | GEO

PAX5 transduction of Pax5-/- pro-B cells

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Pax5-deficient progenitor cell tumors vs wt pro-B-cells

Project description:Comparison of the RNA-expression pattern of Pax5-deficient progenitor-cell tumors with wild-type pro-B-cells Keywords: Genetic modification

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PAX5 transduction of Pax5-/- pro-B cells

Project description:After 5 days in culture with 2ng/ml of IL7, Pax5-/- transduced pro-B cells with PAX5A or PAX5B cDNA were sorted according to their EGFP positivity and total RNA was extracted.

2018-09-01 | GSE104890 | GEO

NIA15k screen. Pax5 +/+, Pax5 -/-, and Rag1-/- pro B cell lines.

Project description:The transcription factor Pax5 is essential for B cell commitment in the mouse, where it represses lineage-inappropriate gene expression, while simultaneously activating the B cell gene expression program. We have performed a global gene expression screen of wild type and Pax5-deficient pro-B cells in an attempt to identify the crucial Pax5 targets in early B-lymphopoiesis. We have also included Rag1-/- and wild type (+/+) proB cells starved of the cytokine IL-7 for 4 hours as controls. Rag1-/- proB cells are incapable of further differentiation due to an absence of immunoglobulin recombination and IL-7 is the major cytokine regulating proB cell growth. Keywords: comparison of genetically modified cell-lines

2007-10-26 | GSE9345 | GEO

Expression data comparing EBF1 versus Pax5 induced genes

Project description:We used microarrays to establish whether EBF1 and Pax5 repress similar or unique genes. We found that EBF1 uniquely represses the expression of the T-lineage transcription factor Gata3. Ebf1-/- pre-pro-B cells were transduced with either control MigR1, MigY-EBF1 or MigR-Pax5 viruses. 24 hours later, RNA was isolated and processed for Affymetrix microarray analysis. Cells expressing EBF1, Pax5, or neither were sorted and processed. Scale represents log2 value of normalized signal level.

2013-04-12 | E-GEOD-46004 | biostudies-arrayexpress

PAX5 promotes heterocharomatin formation

Project description:Heterochromatin has a high density of DNA and low rates of gene transcription.H3k9me3 is a conserved histone modification, and is best known for its role in constitutive heterochromatin formation.H1155, which is a neuroendocrine large cell lung cancer cell line, has a dense nucleus and a high level of occupancy of H3K9me3. PAX5 is a neurogenesis and B lymphocyte development transcription factor, and endogenous expressed in neuroendocrine carcinoma, including H1155 cell line. To assess whether PAX5 promotes heterochromatin formation in H1155, we knock out PAX5 in H1155 cell (KO-PAX5) by CRISPR/Cas9 and use H3K9me3 to performed ChIP-seq.

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IGH analysis in pro-B cells with H3K4me3, H3K4me2, H3K9ac, H3K27me3, Pax5 and CTCF [ChIP-chip]

Project description:VH-DJH recombination of the immunoglobulin heavy-chain (Igh) locus is temporally and spatially controlled during early B-cell development, and yet no regulatory elements other than the VH gene promoters have been identified throughout the entire 2.5-Mb VH gene cluster. Here we discovered novel regulatory sequences that are interspersed in the distal VH gene region. These conserved repeat elements were characterized by the presence of Pax5-dependent active chromatin, the binding of Pax5, E2A, CTCF and Rad21 as well as by Pax5-dependent antisense transcription in pro-B cells. The Pax5-activated intergenic repeat (PAIR) elements were no longer bound by Pax5 in pre-B and B cells consistent with the loss of antisense transcription, whereas E2A and CTCF interacted with PAIR elements throughout early B-cell development. The pro-B-cell-specific and Pax5-dependent activity of the PAIR elements suggests that they are involved in the regulation of distal VH-DJH recombination at the Igh locus. Analysis of chromatin and TF binding in rag2-/- and pax5-/- rag2-/- pro-B cells. Chip-Chip with one experiment for each antibody, 12 samples.

2011-02-11 | E-GEOD-27074 | biostudies-arrayexpress

Expression data comparing EBF1 versus Pax5 induced genes

2013-04-12 | GSE46004 | GEO

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