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Ultrasonic surgical aspirate is a reliable source for culturing glioblastoma stem cells

ABSTRACT: Glioblastoma stem cells (GSCs) are thought to be the source of tumor growth and therapy resistance. Core biosies that are used to generate GSC cultures are ususally taken from one part of the tumor and are thus unlikely to represent intra-tumoral heterogeneity. This study shows that the ultrasonic aspirates (UA), that are usually considered as a biological waste, can be used as a reliable source of GSCs. Furthermore the UA aspirates seem to be capturing the tumorigenic signature better than the traditional biopsies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE84707 | GEO | 2017/02/10

SECONDARY ACCESSION(S): PRJNA330866

REPOSITORIES: GEO

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Ultrasonic surgical aspirate is a reliable source for adult neural progenitor cells (aNPCs)

Project description:Adult neural progenitor cells (aNPCs) are a potential source for cell based therapy for neurodegenerative diseases and traumatic brain injuries. We show that the ultrasonic aspirate samples that are typically considered as a waste after surgery are a great source for aHNPCs.

2017-08-15 | GSE89201 | GEO

Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells.

Project description: Not available

| S-EPMC5015049 | biostudies-literature

FFPE-samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas

Project description:During surgical procedures of gliomas, tissular material obtained from cavitational ultrasonic surgical aspirators (CUSAs) is generally discarded but can exceed amount and quality of certain tumour core resections (TCRs). From archived FFPE-samples, we compared the profiles of somatic mutations and gene expression in the solid material from aspirates and TCRs obtained from the same patients in selected gliomas encompassing grade II to IV. Despite the characteristic heterogeneity of gliomas, we found substantial similarities between the corresponding aspirates and TCRs that included mutations used in the clinical routine and glioma-associated transcriptional signatures. Based on these results, we confirmed the suitability of CUSA-fixed biomaterial from glioma surgeries for downstream applications.

2021-10-20 | GSE166696 | GEO

Rapid DNA methylation-based classification of pediatric brain tumours from ultrasonic aspirate specimens

Project description:Cavitating ultrasonic aspirator devices are frequently used in pediatric neurosurgery for efficient microsurgical resection of brain tumours while minimizing tissue damage to surrounding healthy brain. Within this study molecular diagnostics using methylation-based classification of ultrasonic aspirated samples was performed and performance against routine microarray diagnostics was assessed.

2024-02-08 | GSE255044 | GEO

FFPE-samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas

Project description:FFPE-samples from cavitational ultrasonic surgical aspirates are suitable for RNA profiling of gliomas

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Evaluation of efficacy and safety of an ultrasonic surgical system and reusable shear for ultrasonic surgical system in colorectal surgery: A prospective, single-center, randomized, open, parallel controlled, non-inferiority trial

Project description:Interventions: Experiment Group A:use the ultrasonic surgical systems (Ezisurg Medical Co., Ltd.) in the colorectal surgery;Experiment Group B:use the reusable shear for ultrasonic surgical system (Ezisurg Medical Co., Ltd.) in the colorectal surgery;Control Group:use the Harmonic Ace+7, 5mm Diameter Shears with Advanced Hemostasis (Ethicon Endo-Surgery,LLC) in the colorectal surgery Primary outcome(s): overall good performance rate Study Design: Parallel

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Evaluation of chromosomal abnormalities and copy number variations in fetuses with ultrasonic soft markers

Project description:This study aimed to evaluate the clinical value of copy number variations (CNVs) in fetuses with ultrasonic soft markers. Among 1131 fetuses, 729 had single ultrasonic soft marker, 322 had two ultrasonic soft markers, and 80 had three or more ultrasonic soft markers. All fetuses underwent single nucleotide polymorphism (SNP) array analysis. Among 1131 fetuses with ultrasonic soft markers, 46 had chromosomal abnormalities. In addition to the 46 fetuses with chromosomal abnormalities consistent with the results of the karyotyping analysis, the SNP array identified additional 6.1% (69/1131) abnormal CNVs. No significant difference was found in the rate of abnormal CNVs among the groups. The SNP array can fully complement conventional karyotyping in fetuses with ultrasonic soft markers, improve detection rate of chromosomal abnormalities, and affect pregnancy outcomes.

2020-12-24 | GSE163799 | GEO

Tracheal aspirate RNA sequencing identifies distinct immunological features of COVID-19 ARDS

Project description:We compared differential gene expression in tracheal aspirates collected mechanically ventilated subjects with COVID-19 ARDS to gene expression in tracheal aspirates from: 1) subjects with ARDS from other casues and 2) mechanically ventilated controls without evidence of pulmonary disease.

2020-12-18 | GSE163426 | GEO

Human colon aspirate proteins analyzed by LC-MSMS

Project description:Colonic aspirates were collected at diagnostic colonoscopy from inflammatory bowel disease (IBD) and control, treatment-naive children. The colonic mucosal-luminal interface (MLI) proteomes were analyzed for 18 control and 42 IBD patients by liquid-chromatography mass spectrometry.

2018-03-19 | PXD006383 | Pride

Tracheal aspirate transcriptomic and miRNA signatures of extreme premature birth with bronchopulmonary dysplasia

Project description:We analyzed mRNA profiles in tracheal aspirates from 53 newborns receiving invasive mechanical ventilation. Twenty-six infants were extremely preterm diagnosed with BPD and twenty-seven were term babies receiving invasive mechanical ventilation for elective procedure. Specific mRNA signatures in TAs may serve as potential biomarkers for extreme prematurity and BPD pathogenesis.

2020-11-08 | GSE156028 | GEO

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