Transcriptomics,Genomics

Dataset Information

152

Paf1 modulates Piwi/piRNA silencing capacity in Drosophila


ABSTRACT: To test whether the RNA Polymerase II factor PAF1 affects PIWI silencing, we examined the transcriptome expression levels in Drosophila OSS cells following siRNA knockdown of these factors. Overall design: The total RNAs from OSS cells were extracted after siRNA knockdown of GFP negative control, PAF1, PIWI, and a double knockdown of both PAF1 and PIWI. The RNAs were then converted into strand-specific sequencing libraries with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina.

REANALYSED by: GSE117217

INSTRUMENT(S): Illumina NextSeq 500 (Drosophila melanogaster)

SUBMITTER: NELSON LAU  

PROVIDER: GSE84766 | GEO | 2017-08-31

SECONDARY ACCESSION(S): PRJNA331099

REPOSITORIES: GEO

altmetric image

Publications

Drosophila PAF1 Modulates PIWI/piRNA Silencing Capacity.

Clark Josef P JP   Rahman Reazur R   Yang Nachen N   Yang Linda H LH   Lau Nelson C NC  

Current biology : CB 20170824 17


To test the directness of factors in initiating PIWI-directed gene silencing, we employed a Piwi-interacting RNA (piRNA)-targeted reporter assay in Drosophila ovary somatic sheet (OSS) cells [1]. This assay confirmed direct silencing roles for piRNA biogenesis factors and PIWI-associated factors [2-12] but suggested that chromatin-modifying proteins may act downstream of the initial silencing event. Our data also revealed that RNA-polymerase-II-associated proteins like PAF1 and RTF1 antagonize P  ...[more]

Similar Datasets

2013-02-11 | E-GEOD-43829 | ArrayExpress
2007-10-24 | E-GEOD-9138 | ArrayExpress
2007-10-25 | GSE9138 | GEO
| GSE74097 | GEO
2015-08-13 | E-GEOD-70408 | ArrayExpress
2015-12-04 | E-GEOD-74423 | ArrayExpress
2010-06-12 | E-GEOD-21763 | ArrayExpress
| GSE74423 | GEO
2010-06-13 | GSE21763 | GEO
2007-09-19 | E-GEOD-3200 | ArrayExpress