Transcriptomics,Genomics

Dataset Information

42

Molecular basis of drought-induced susceptibility to the rice blast fungus Magnaporthe oryzae


ABSTRACT: In order to understand the mechanisms of Drought induced susceptibility (DIS) we’ve conducted a dual RNAseq experiment on rice infected tissues by Magnaporthe oryzae. At 4 days post inoculation tissues have been collected on mock inoculated and M. oryzae inoculated plants. Rice were conducted under two type of water regime: DIS Drought during three days before inoculation, NoDIS no drought before inoculation. RNAseq was conducted both on rice and fungal RNA. Overall design: shoot tissus mRNA 21-day old rice plant were generated by deep sequencing. Three samples per conditions, Four conditions: NoDIS Mock, NoDIS Inoc, DIS Mock, DIS Inoc. NoDIS = plant were conducted with a normal water regime. DIS = plant received no watering during three days before inoculation. Mock = Plant inoculated with control solution (gelatin 0,5%). Inoc = Plant inoculated with Magnaporthe oryzae isolate Fr13 with gelatin 0,5%.

INSTRUMENT(S): Illumina HiSeq 2000 (Oryza sativa)

SUBMITTER: Elsa Ballini  

PROVIDER: GSE84800 | GEO | 2016-10-01

SECONDARY ACCESSION(S): PRJNA331158

REPOSITORIES: GEO

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Publications

Transcriptional Basis of Drought-Induced Susceptibility to the Rice Blast Fungus Magnaporthe oryzae.

Bidzinski Przemyslaw P   Ballini Elsa E   Ducasse Aurélie A   Michel Corinne C   Zuluaga Paola P   Genga Annamaria A   Chiozzotto Remo R   Morel Jean-Benoit JB  

Frontiers in plant science 20161027


Plants are often facing several stresses simultaneously. Understanding how they react and the way pathogens adapt to such combinational stresses is poorly documented. Here, we developed an experimental system mimicking field intermittent drought on rice followed by inoculation by the pathogenic fungus Magnaporthe oryzae. This experimental system triggers an enhancement of susceptibility that could be correlated with the dampening of several aspects of plant immunity, namely the oxidative burst a  ...[more]

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