Transcriptomics,Genomics

Dataset Information

160

Dirofilaria immitis exhibits sex- and stage-specific differences in excretory/secretory miRNA profiles in vitro


ABSTRACT: We report analyses of the types, amounts and stage-dependence of microRNAsfound in D. immitis culture media recovered after incubating 800,000 microfilariae for 6 days, 500 third-stage larvae (L3) and 500 fourth-stage larvae (L4) for 7 days, as well as 40 adult females and 40 adult males for 48 hours, all separately. Overall design: Examination of excretory/secretory miRNAs in 5 different stages of Dirofilaria immitis released in culture media.

INSTRUMENT(S): Illumina HiSeq 2000 (Dirofilaria immitis)

SUBMITTER: Lucienne Tritten  

PROVIDER: GSE84866 | GEO | 2017-01-16

SECONDARY ACCESSION(S): PRJNA335393

REPOSITORIES: GEO

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Publications

Dirofilaria immitis exhibits sex- and stage-specific differences in excretory/secretory miRNA and protein profiles.

Tritten Lucienne L   Clarke Damian D   Timmins Scott S   McTier Tom T   Geary Timothy G TG  

Veterinary parasitology 20161109


The canine heartworm Dirofilaria immitis releases excretory/secretory molecules into its host and in culture. We report analyses of the types, amounts and stage-dependence of microRNAs and proteins found in D. immitis culture media recovered after incubating 800,000 microfilariae for 6days, 500L3 and 500L4 for 7days, as well as 40 adult females and 40 adult males for 48h, all separately. In addition, the presence of exosome-like particles was established by nanoparticle tracking analysis. Our re  ...[more]

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