Genomics

Dataset Information

22

Cdx and T Brachyury co-activate growth signaling in the embryonic axial progenitor niche.


ABSTRACT: In vertebrate embryos, anterior tissues are generated early, followed by the other axial structures that emerge sequentially from a posterior growth zone. The genetic network driving posterior axial elongation in mice, and its disturbance in mutants with posterior truncation are not yet fully understood. We show that the combined expression of Cdx2 and T Brachyury is essential to establish the core signature of posterior axial progenitors. Cdx2 and T Brachyury are required for extension of a similar trunk portion of the axis. Simultaneous loss of function of these two genes disrupts axial elongation to a much greater extent than each single mutation alone. We identify and validate common targets for Cdx2 and T Brachyury in vivo including Wnt and Fgf pathway components active in the axial progenitor niche. Our data demonstrate that integration of the Cdx/Hox and T Brachyury transcriptional networks controls differential axial growth during vertebrate trunk elongation. Overall design: ChIP-seq for several transcription factors and histone mark H3K27ac, ATAC-seq and RNA-seq in Epiblast stem cells (EpiSCs) and early stage embryos.

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Jacqueline Deschamps  

PROVIDER: GSE84899 | GEO | 2016-12-20

SECONDARY ACCESSION(S): PRJNA335589

REPOSITORIES: GEO

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Publications

Cdx and T Brachyury Co-activate Growth Signaling in the Embryonic Axial Progenitor Niche.

Amin Shilu S   Neijts Roel R   Simmini Salvatore S   van Rooijen Carina C   Tan Sander C SC   Kester Lennart L   van Oudenaarden Alexander A   Creyghton Menno P MP   Deschamps Jacqueline J  

Cell reports 20161201 12


In vertebrate embryos, anterior tissues are generated early, followed by the other axial structures that emerge sequentially from a posterior growth zone. The genetic network driving posterior axial elongation in mice, and its disturbance in mutants with posterior truncation, is not yet fully understood. Here, we show that the combined expression of Cdx2 and T Brachyury is essential to establish the core signature of posterior axial progenitors. Cdx2 and T Brachyury are required for extension of  ...[more]

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