Transcriptomics,Genomics

Dataset Information

45

Increased osteoblast viability at alkaline pH in vitro provides a new perspective on bone regeneration


ABSTRACT: Developing osteoblasts undergo a sequence of three consecutive phases: cell proliferation, extracellular matrix maturation, and mineralization. We investigated pH effects on these phases using the osteoblast-like cell line MC3T3-E1. Overall design: MC3T3sc14 cells were cultured at pH 7.4, 7.8 or 8.4 for 14 days before gene expression analysis was run with Affymetrix GeneChip Mouse 2.0 ST Arrays (4 samples each)

INSTRUMENT(S): [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Alexander Rebl  

PROVIDER: GSE84907 | GEO | 2016-12-28

SECONDARY ACCESSION(S): PRJNA335615

REPOSITORIES: GEO

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Publications

MC3T3 osteoblast-like cells cultured at alkaline pH: Microarray data (Affymetrix GeneChip Mouse 2.0 ST).

Galow Anne-Marie AM   Rebl Alexander A   Koczan Dirk D   Gimsa Jan J  

Data in brief 20170510


It is well known that pH plays a pivotal role in the control of bone remodeling. However, no comprehensive gene expression data are available for the effects of alkaline pH on osteoblasts. We cultured differentiating MC3T3-E1 osteoblast-like cells at pH 7.4, 7.8, and 8.4 for 14 days. To identify differential gene expression, microarray data were collected with Affymetrix GeneChips. The data were validated by real-time PCRs for five genes that were found to be greatly regulated in the GeneChip-ex  ...[more]

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