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MRNA sequencing in acute myeloid leukemia (AML) cells with and without knockdown of FTO

ABSTRACT: To identify the expression of mRNAs after knockdown of FTO, we performed RNA-Seq in MA9.3ITD cells with or without knockdown of FTO.

ORGANISM(S): Homo sapiens

PROVIDER: GSE85008 | GEO | 2016/12/20

SECONDARY ACCESSION(S): PRJNA335858

REPOSITORIES: GEO

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5hmC-seq in human MonoMac6 acute myeloid leukemia (AML) cells with and without knockdown of TET1

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RNA seq and MeRIP-seq of COV434 cells before and after FTO knockdown

Project description:Multifunctional N6-methyladenosine (m6A) has been revealed to be an important epigenetic component in various physiological and pathological processes, but its role in female ovarian aging remains unclear. Thus, we demonstrated m6A demethylase FTO downregulation and the ensuing increased m6A in granulosa cells (GCs) of human aged ovaries, while FTO-knockdown GCs showed faster aging-related phenotypes mediated. Using the m6A-RNA-sequence technique (m6A-seq), increased m6A was found in the FOS-mRNA-3'UTR, which is suggested to be an erasing target of FTO that slows the degradation of FOS-mRNA to upregulate FOS expression in GCs, eventually resulting in GC-mediated ovarian aging. FTO acts as a senescence-retarding protein via m6A, and FOS knockdown significantly alleviates the aging of FTO-knockdown GCs.

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Effect of FTO overexpression and knockdown on transcriptome of HEK293 cells

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