Transcriptomics,Genomics

Dataset Information

144

Genome-wide expression profiling of Neofusicoccum parvum virulence factors in response to different growth substrates in vitro and during an interaction time course with grapevine stems


ABSTRACT: Transcriptome profiling by RNA sequencing determined the genome-wide patterns of expression of N. parvum virulence factors when PDA or grape wood were provided as nutrient source and during an extensive interaction time course with grapevine stem. Overall design: N. parvum UCD646So (ATCC number: MYA-3706) was grown on Potato Dextrose Agar (PDA) or agar containing ground grapevine wood (V. vinifera cv. Cabernet Sauvignon clone FPS 08 and V. vinifera cv. Merlot clone FPS 03). Potted V. vinifera cv. Cabernet Sauvignon clone FPS 19 plants were inoculated with N. parvum UCD646So mycelia. Stems were collected at seven time points: 0 hour post-inoculation (hpi), 3 hpi, 24 hpi, 2 weeks post-inoculation (wpi), 6 wpi, 8 wpi, and 12 wpi from 1 cm below the inoculation site. Three biological replicates were collected from each medium and for each infection time point.

INSTRUMENT(S): Illumina HiSeq 3000 (Neofusicoccum parvum)

SUBMITTER: Mélanie Massonnet  

PROVIDER: GSE85079 | GEO | 2017-03-08

SECONDARY ACCESSION(S): PRJNA336064

REPOSITORIES: GEO

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Publications

Condition-dependent co-regulation of genomic clusters of virulence factors in the grapevine trunk pathogen Neofusicoccum parvum.

Massonnet Mélanie M   Morales-Cruz Abraham A   Figueroa-Balderas Rosa R   Lawrence Daniel P DP   Baumgartner Kendra K   Cantu Dario D  

Molecular plant pathology 20161204 1


The ascomycete Neofusicoccum parvum, one of the causal agents of Botryosphaeria dieback, is a destructive wood-infecting fungus and a serious threat to grape production worldwide. The capability to colonize woody tissue, combined with the secretion of phytotoxic compounds, is thought to underlie its pathogenicity and virulence. Here, we describe the repertoire of virulence factors and their transcriptional dynamics as the fungus feeds on different substrates and colonizes the woody stem. We asse  ...[more]

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