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Transcriptional profiling of GDF11 or TGFB1 stimulated NMuMG 3D spheroids

ABSTRACT: The objective of this study was to identify transcriptional changes differentially regulated by GDF11 stimulation compared to TGFB1 Microarrays were used to capture the differential transcriptional response to acute GDF11 stimulation compared to acute TGFB1 stimulation

ORGANISM(S): Mus musculus

PROVIDER: GSE85224 | GEO | 2018/02/13

SECONDARY ACCESSION(S): PRJNA336578

REPOSITORIES: GEO

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Effect of GDF11 on RANKL-induced osteoclastogenesis

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2016-07-21 | E-GEOD-84609 | biostudies-arrayexpress

RNAseq of wild type and GDF11-/- Neuro-2a cells

Project description:Growth differentiation factor 11 (GDF11), is one of the members of transforming growth factor β (TGFβ) superfamily. We set out to unequivocally reveal the effect of endogenous GDF11 on biological process by adopting CRISPR/Cas9 gene knockout strategy to specifically delete GDF11 gene in Neuro-2a cells. We analyze mRNA profiles of differentially genes in wild type (WT) and GDF11 knockout (GDF11-/-) Neuro-2a cells by using RNA-seq technology. The RNA-seq data reported here provide a fundamental materials and evidence for investigations of biological function of GDF11.

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Effect of GDF11 on RANKL-induced osteoclastogenesis

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Restoring Systemic GDF11 Levels Reverses Age-Related Dysfunction in Mouse Skeletal Muscle

Project description:In this study, we investigated signaling pathways in Skeletal muscle precursors that are altered with aging and age-related deficits in muscle regenerative potential. We performed fluorescence activated cell sorting (FACS) to obtain highly purified skeletal muscle satellite cells from young, middle-aged and old mice. Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting that young blood contains humoral "rejuvenating" factors that can restore regenerative function. Here, we demonstrate that the circulating protein growth differentiation factor 11 (GDF11) is a rejuvenating factor for skeletal muscle. Supplementation of systemic GDF11 levels, which normally decline with age, by heterochronic parabiosis or systemic delivery of recombinant protein, reversed functional impairments and restored genomic integrity in aged muscle stem cells (satellite cells). Increased GDF11 levels in aged mice also improved muscle structural and functional features and increased strength and endurance exercise capacity. These data indicate that GDF11 systemically regulates muscle aging and may be therapeutically useful for reversing age-related skeletal muscle and stem cell dysfunction. We used Affymetrix Mouse Genome array to identify global transcriptional changes associated with age in skeletal muscle precursors.

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