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Gene expression profiling in Human mammary epithelial cell (MCF10A) by repression of KRT81 expression

ABSTRACT: To investigate new health functions of hair keratin KRT81 in MCF10A, we compare altered of the genes expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE85236 | GEO | 2016/08/06

SECONDARY ACCESSION(S): PRJNA337911

REPOSITORIES: GEO

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Gene expression profiling in Human mammary epithelial cell (MCF10A) by repression of KRT81 expression

Project description:To investigate new health functions of hair keratin KRT81 in MCF10A, we compare altered of the genes expression. The expression of KRT81 was blocked by transient transfection with KRT81 siRNA in MCF10A cells.

2016-08-06 | E-GEOD-85236 | biostudies-arrayexpress

Effects of black currant extract on gene expression on MCF10A

Project description:To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (50μg/ml) treatment in MCF10A.

2016-08-06 | GSE85235 | GEO

Effects of black currant extract on gene expression on MCF10A

Project description:To investigate new health functions by blackcurrant extract (BCE), we compare altered of the genes expression before and after BCE (50Î¼g/ml) treatment in MCF10A. MCF10A cells were seeded in culture dish and maintain to confluent. Then medium replace with mammary epithelial cell growth medium kit with or without BCE (50Î¼g/ml). After the cells were incubated for 24 h at 37 Â°C 5% CO2.

2016-08-06 | E-GEOD-85235 | biostudies-arrayexpress

Differential microRNA expression in adherent versus suspended MCF10A mammary epithelial cells

Project description:The ability to survive following loss of adhesion underpins cancer metastasis and significantly contributes triple negative breast cancer (TNBC) morbidity. Epithelial cells that form solid tumors must evade anoikis, a special type of cell death that occurs upon detachment from the extracellular matrix in order to colonize in distal sites. While there have been several advances in the understanding of the mechanisms by which cells undergo anoikis, the role for noncoding RNAs (ncRNAs) in this process is less well understood. An important role for microRNAs (miRNAs) in regulating anoikis is beginning to emerge, however in general the mechanisms by which miRNAs regulate anoikis are largely unknown. Thus we utilized the anoikis-sensitive immortalized mammary epithelial cell line, MCF10A to unbiasedly determine the microRNAs differentially regulated upon detachment.

2023-10-30 | GSE244857 | GEO

Snail and Twist expression in HMLE and MCF10A cells

Project description:Snail and Twist are two EMT inducer, expression of Snail or Twist will induce EMT in HMLE and MCF10A cells. By introducing Snail or Twist in HMLE and MCF10A cells, which lack the expression of these two proteins, will identify the genes are induced during EMT. We used microarray analysis to compare the gene expression profiles between the mammamry epithleial cells and the cells undergone EMT.

2020-01-10 | GSE143349 | GEO

Regulation of gene expression by loss-of-YAP/TAZ in MCF10A mammary epithelial cells

Project description:YAP1 (Yes-associated protein 1) and TAZ (transcriptional coactivator with PDZ-binding motif, or WWTR1) are nucleo-cytoplasmic shuttling proteins that can function in the nucleus as transcriptional coactivators. Their role in regulating gene transcription has been so far mainly investigated by overexpressing YAP1 or TAZ, while here we sought to determine which genes are regulated by endogenous levels of YAP/TAZ. To this end, we compared MCF10A cells transfected with a control non-targeting siRNA to cells transfected with two independent mixes of siRNA targeting both YAP and TAZ.

2015-03-23 | GSE59229 | GEO

Gene expression profiles induced by overexpression of PDEF in MCF10A mammary epithelial cell line

Project description:Recent studies suggest that PDEF is required for secretory cell differentiation in several epithelial tissues. To investigate PDEF in the mammary gland, we examined the effect of this transcription factor on gene expression using microarray based profiling of MCF-10A cells. These cells are non-transformed mammary epithelial cells that express protein and gene expression programs of basal epithelial cells and undetectable levels of endogenous PDEF. Bioinformatics analysis of the genes induced or repressed by PDEF overexpression in MCF10A cells revealed a striking effect on expression of luminal and myoepithelial cell markers.

2014-03-29 | GSE40986 | GEO

Expression profiling in cohesin mutant MCF10A epithelial and CMK leukaemia cells

Project description:MCF10A cells were CRISPR-Cas9 edited to create heterozygous deletion in RAD21 and SMC3 subunits of cohesin. STAG2 is on the X chromosome, hence CRISPR-Cas9 editing resulted in complete loss of STAG2. Total RNA was sequenced from the MCF10A parental and cohesin mutant MCF10A lines. The acute megakaryoblastic leukaemia cell line CMK was CRISPR-Cas9 edited to cotain STAG2 R614* mutation. CRISPR-Cas9 edited STAG2 mutant line showed complete loss of STAG2. CMK parental and the STAG2 mutant line were treated with Wnt3a for 4 hours and total RNA was sequenced at in the control or non-treated (con) and following 4 hours of Wnt3a treatment (Wnt3a4hr).

2020-12-13 | GSE154086 | GEO

Short and long read sequencing of human mammary epithelial MCF10a-Snail-ER cells after Epithelial-to-Mesenchymal Transition initiation

Project description:We cultured MCF10a-Snail-ER cells and induced EMT initiation with tamoxifen. A matched sequencing of their PolyA RNA was performed, using Illumina and direct RNA Oxford Nanopore sequencing technologies. Both generated datasets supported the development of hybrid bioinformatics tools.

2021-09-09 | GSE126638 | GEO

GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A

Project description:Excessive expression of subunit 1 of GIRK1 in ER+ breast tumors is associated with reduced survival times, and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells overexpressing GIRK1 were engineered, neoplasia associated vital parameters and resting potentials were measured and compared to controls. Presence of GIRK1 resulted in resting potentials negative to controls. Upon GIRK1 overexpression, several cellular pathways were sizably regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10AGIRK1 with control (MCF10AeGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extracellular matrix interactions were impaired. Vital parameters in MCF7 cells were affected akin the benign MCF10A lines, although to a lesser extent. Thus, GIRK1 overexpression will aid in classification of ER+ breast tumors and bears the potential to open a new window for the therapy of this detrimental disease.

2019-10-01 | GSE138155 | GEO

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