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ACF1 regulates nucleosome spacing in Drosophila euchromatin [MNase-seq BG3]

ABSTRACT: The nature of chromatin as regular succession of nucleosomes has gained iconic status. The average nucleosome repeat length (NRL) determined by classical means serves as index for bulk chromatin of a given specimen. However, this value is dominated by regular heterochromatin since nucleosomal arrays are often not regular at individual single copy sequences. To obtain a measure for nucleosome regularity in euchromatin we subjected nucleosome dyad profiles to autocorrelation and spectral density analyses. This revealed variation in nucleosome regularity and NRL at different types of euchromatin and yielded a comprehensive catalog of regular phased nucleosome arrays (PNA). The absence of the nucleosome sliding factor ACF1 correlated with global loss of regularity in euchromatin and increased NRL and compromised phasing at a novel type of PNA. Our approach is generally applicable to characterize hallmarks of euchromatin organization.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE85406 | GEO | 2018/01/01

SECONDARY ACCESSION(S): PRJNA338369

REPOSITORIES: GEO

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Drosophila melanogaster

Project description:ACF1 regulates nucleosome spacing in Drosophila euchromatin [ChIP-seq]

| PRJNA338341 | ENA

Drosophila melanogaster

Project description:ACF1 regulates nucleosome spacing in Drosophila euchromatin [MNase-seq BG3]

| PRJNA338369 | ENA

Drosophila melanogaster

Project description:ACF1 regulates nucleosome spacing in Drosophila euchromatin [Mnase-seq embryos]

| PRJNA338368 | ENA