Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The mechanism of restoration ovarian function using female germline stem cell

ABSTRACT: Germ stem cells are the only stem cells which can produce gametes in vivo. Recently, accumulating evidence demonstrates the isolation and culture of female germline stem cells (FGSCs) from adult mice and humans. Here, we established FGSCs line from single EGFP transgenetic mouse, and traced their differentiation in vivo. Then we compared the follile development from transplanted FGSCs mice (F-TF) with that from wide type mice (F-WT) by RNA-Seq. qRT–PCR validation was performed using SYBR Green assays. Using Illumina HiSeq2500 sequencer, we generated 51.5 Gb of sequencing data from the 8 samples. On average, we detected expression of 20 966 transcripts. Under the criteria fold change > 2 or < 0.5, we obtained 7005 and 5764 differential expressed genes (DEGs) from F-TF or F-WT,respectively. Weighted correlation network analysis (WGCNA) analysis identified core genes and two potential networks during folliculogenesis which were mainly down-regulated after preantral follicle. This study was the first to analyze the development of transplanted FGSCs progressively and would provide the theoretical basis for clinical translational research of FGSCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE85412 | GEO | 2017/09/08

SECONDARY ACCESSION(S): PRJNA338393

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression data from anti-müllerian hormone treated preantral/small antral mouse ovary follicles

2015-05-01 | GSE56737 | GEO

Expression data from anti-müllerian hormone treated preantral/small antral mouse ovary follicles

2015-05-01 | E-GEOD-56737 | biostudies-arrayexpress

Glycopeptide glycoforms analysis of mouse transferrin

Project description:Collagen type II-induced arthritis (CIA) is a disease, which includes inflammatory and autoimmune reactions. Spleen protein extracts were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analysis to identify proteins that were different in concentration ally expressed in CD38-KO and B6 WT mice with arthritis or with inflammation. Using multivariate analyses, in Col-II-immunized mice 20 differentially expressed spleen protein species were able to discriminate between WT and CD38-KO mice. Among them, multiple serotransferrin (Tf) species were identified. In contrast, in CFA/IFA-treated mice, the distinct protein profile, which discriminates between CD38-KO and WT mice, was unrelated with Tf. Unexpectedly, non-immunized CD38-KO mice showed a distinct proteome profile as compared with that in non-immunized WT mice, and again multiple protein species were identified as Tf. By using a LC-TOF-MS method to separate and detect Tf glycopeptide glycoforms in serum samples, differences in the Tf glycosylation pattern between CD38-KO and WT mice sera were found in both non-immunized and CIA+ mice. Data on Tf spots separated by 2-DE indicate differences in glycosylation related with NeuGc content. In summary, Tf changed significantly in its glycosylation pattern in mice with arthritis

2015-12-10 | PXD003163 | Pride

Distribution and function of 3',5'-Cyclic-AMP hydrolysing phosphodiesterases in the human ovary

Project description:The aim of the study was to describe and characterise the phosphodiesterases in the human ovary. Part of the study included results from analysis of mRNA microarray data from follicles and granulosa cells from three previously published studies(E-MEXP-3783, E-MTAB-2203, E-MTAB-1670). In addition, we used data from two unpublished studies with granulosa cells isolated from 4-6 mm antral follicles and preantral follicles. The included studies covered the folliculogenesis from the preantral stage to after induction of ovulation. For comparison, previously published dataset from heart (E-GEOD-22253, E-MEXP-2654), parietal cortex (E-GEOD-35977), cerebellum (E-GEOD-35974), lung (E-GEOD-43458), and peripheral blood mononucleated cells(E-GEOD-23832) were included in addition to various tissues from Affymetrix's sample data set.

2014-10-31 | E-MTAB-2862 | biostudies-arrayexpress

mass spectrum data for the manuscript "High-throughput quantitative proteomic analysis uncovers the protein-expression profiles during hormone-dependent folliculogenesis in mice"

Project description:To describe the proteome of ovarian follicles in mice from hormone-independent stage to hormone-dependent stage, we isolated thousands of secondary follicles, preantral follicles and antral follicles and performed mass spectrometry (MS) to identify potential regulators during folliculogenesis

2022-03-08 | PXD032115 | iProX

Expression of TGFbeta Superfamily Growth Factors their Receptors and the Associated SMADs in Five Isolated Size matched Populations of Preantral Follicles from Normal Human Ovaries

Project description:In mammals, members of the transforming growth factor-beta (TGF-beta) superfamily are known to have key roles in the regulation of follicular growth and development. The aim of the study was to evaluate the expression of TGF-beta superfamily growth factors, their receptors and downstream SMAD signalling molecules during early human folliculogenesis. Human preantral follicles were enzymatically isolated from surplus ovarian tissue obtained from women having ovarian cortical tissue frozen for fertility preservation. A total of 348 human preantral follicles, ranging from 40 to 200 um in diameter, were isolated from ovarian tissue obtained from 15 women, aged 24M-^V34 years. Isolated preantral follicles were grouped according to diameter in five size-matched populations spanning the primordial, primary and secondary stage follicles, and analyzed by whole-genome microarray analysis. Selected proteins/genes were analysed by immunocytochemistry and quantitative RT-PCR. TGF-beta superfamily genes with overall highest mRNA expressions levels included growth differentiation factors 9 (GDF9), bone morphogenic protein-15 (BMP15), BMP6, BMP-receptor-2 (BMPR2), anti-MM-|llerian hormone receptor 2 (AMHR2), TGFbetaR3, inhibin-alpha (INHA), and intracellular SMAD3 and SMAD4. Moreover, genes which were differentially expressed from the primordial to the late secondary stage follicles included GDF9 (p<0.01), BMP15 (p<0.001), AMH (p<0.0001), INHBB (p<0.05), TGFbetaR3 (p<0.05) and SMAD4 (p<0.05). Collectively, these data indicate that the active TGF-beta superfamily pathways in early human folliculogenesis consist of primarily GDF9 combined with synergistic effects of BMP15 through the BMPR2 and intracellular activation of SMAD3 and SMAD4, and that AMH and Inhibin B are engaged in intrafollicular events from the onset of follicular growth.

2013-08-06 | E-MEXP-3783 | biostudies-arrayexpress

Gene expression data from anti-müllerian hormone (AMH) treated mouse ovary follicles

2015-05-01 | GSE63907 | GEO

Gene expression data from anti-müllerian hormone (AMH) treated mouse ovary follicles

Project description:Anti-müllerian hormone (AMH) has an inhibitory effect on ovarian follicle development. However, the mechanism by which AMH regulates folliculogenesis remains to be elucidated. In this study we aimed to investigate the changes in transcriptome of preantral to small antral mice follicles after culturing with AMH and thereby identify candidate genes to be involved. This study is a repetition of a previous study (GEO accession .no.: GSE56737) at the concentration of 50 ng/mL. The overall design are almost similar to GSE56737 except that it was performed in quadruplicate. Preantral to small antral follicles were collected from dissected ovaries from six to eight weeks old female C57BL/6Tac mice. Twelve hour as well as 24 hour experiments were performed in one fixed concentration of AMH and an untreated control. The experiments were performed in quadroplicate. In total 16 samples: 2 x conc. (control and 50 ng/ml AMH), 2 x time points, 4 x experiments (quadroplicate). Samples are pairwise comparable within each experiment, that is, sample 1-2 or 3-4 or 5-6 or 7-8 or 9-10 or 11-12 or 13-14 or 15-16. Data are also comparable groupwise; (1,5,9,13) vs (2,6,10,14) or (3,7,11,15) vs (4,8,12,16).

2015-05-01 | E-GEOD-63907 | biostudies-arrayexpress

Genome wide analysis of STAT3-mediated transcription and binding sites in mouse female germ stem cell (ChIP-seq)

Project description:Activation of JAK-STAT3 signaling by leukemia inhibitory factor (LIF) is required for maintaining self-renewal of mouse embryonic stem cells (mESCs). STAT3 perform cell type-specific roles in different cell type, here we revisit the role of STAT3 using mouse female germ stem cell (mFGSCs). We applied CRISPR/Cas9 system to generate Stat3 knockout FGSCs and then observed cell growth inhibition and cell cycle arrest in KO cell line. By combining genome wide ChIP-Seq and RNA-Seq, we identified 5990 STAT3 binding sites and discovered serval genes specific regulated by STAT3 that were involved in stem cell proliferation and female gonad development in FGSCs. In general, we identify key roles of STAT3 for sustains self-renewal and proliferation for FGSCs in this study.

2017-12-20 | E-MTAB-6163 | biostudies-arrayexpress

Genome wide analysis of STAT3-mediated transcription and binding sites in mouse female germ stem cell (RNA-seq)

2017-12-20 | E-MTAB-6162 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data