Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of CD122HI versus CD122LO Vγ7+ murine small intestinal IEL at 14-17 days of age

ABSTRACT: Background: The neonatal (D14-17) murine small intestinal epithelium is enriched for Vγ7+ IEL displaying an ‘immature’ CD122LO cell surface phenotype. We believe these cells are precursors for signature CD122HI Vγ7+ IEL that predominate among small intestinal IEL from postnatal D21. Method: To further characterise this potential precursor-product relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq. Conclusion: ~3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL

ORGANISM(S): Mus musculus

PROVIDER: GSE85422 | GEO | 2016/08/11

SECONDARY ACCESSION(S): PRJNA338434

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression analysis of CD122HI versus CD122LO VÎ³7+ murine small intestinal IEL at 14-17 days of age

Project description:Background: Recently have found that Btnl1 expressed by murine enterocytes shapes the local TCR-VÎ³7+ compartment by driving their clonotypic expansion and phenotypic maturation from CD122LO to CD122HI VÎ³7+ cells. The neonatal (D14-17) murine small intestinal epithelium is enriched for VÎ³7+ IEL displaying an â??immatureâ?? CD122LO cell surface phenotype. We believe these cells are precursors for CD122HI VÎ³7+ IEL. Method: To further characterise this putative product-progenitor relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq. Conclusion: >3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL 4 independent Paired samples of CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from the small intestinal epithelium of pooled 14-17 day old mice. RNA was isolated from sorted samples. Gene expression analysis was performed by total RNAseq.

2016-08-11 | E-GEOD-85422 | biostudies-arrayexpress

RNA Sequencing Gene Expression Profile of IEL Incubated In The Presence or Absence of Osteopontin

Project description:We report the application of RNA-Seq technology for high-throughput gene expression profiling of intestinal intraepithelial lymphocytes (IEL) that were incubated for 24 hrs in the presence or absence of recombinant osteopontin.

2018-11-01 | GSE122007 | GEO

Gene expression in WT and LRF-deficient thymic IEL precusors (IELp) and their progeny CD8aa IEL

Project description:Gene expression of IELp and CD8aa IEL was determined by RNAseq

2022-03-30 | GSE149943 | GEO

ScRNAseq of human IEL and LPL T lymphocytes

Project description:We sequenced T cells from 2 control and 2 Crohn's disease patients We determined that in human Th17 T cells are highly enriched in the IEL compartment We identified discrete populations of CD8 and CD4 T cells, which are differently represented in IEL and LP

2021-02-23 | GSE157477 | GEO

Microarray Analysis of Murine IEL Subsets

2007-04-03 | GSE5355 | GEO

Anti-CD3-induced activation of intestinal intraepithelial lymphocytes (IEL)

Project description:The experiment was designed to assess whether conventional and unconventional IEL differ in their response to activation.

2017-03-31 | GSE63368 | GEO

Single-cell Gene expression of WT CD8aa IEL and LRF-deficient CD8aa splenocytes

Project description:To examine how the cluster composition of CD8aa IEL and their transcriptomic signatures were affected by LRF disruption, single-cell gene expression of CD8aa IEL from control (Cd4 Cre–Lrffl/fl) and CD8aa splenocytes from LRF KO (Cd4 Cre+Lrffl/fl ) mice were determined by scRNAseq.

2022-03-30 | GSE186164 | GEO

Comparative analysis of chicken Intestinal Intraepithelial lymphocytes (IEL) following Eimeria acervulina (EA), E. maxima (EM), or E. tenella (ET) infection

Project description:Relative expression levels of mRNAs in chicken IEL experimentally infected with EA, EM, or ET were measured at 1 to 6 days post-infection (dpi) following primary and secondary infections. One week-old chickens were uninfected (negative control) or were orally inoculated with sporulated oocysts of EA, EM, or ET. One week later, the infected chickens were challenged with an identical inoculum of the homologous parasite. Intestinal samples were collected daily from 5 birds in a treatment group at from 1 to 6 dpi following primary and secondary infections. Cecum, duodenum, and jejunum were collected from the birds challenged with E. acervulina, E. maxima, and E. tenella, respectively. Uninfected control samples and one of the 3 infection group samples were labeled with different fluorescent dyes and hybridized simultaneously on the same slide using a reference design with a dye swap protocol. Thirty seven-condition experiment, Non-infected control vs. Primary or secondary EA, EM, or ET infected IEL at 1 to 6. Biological replicates: 2 replicates with dye-switching from each infection groups. Two replicates per array.

2011-08-14 | E-GEOD-31213 | biostudies-arrayexpress

Transcription profiling of dfferentiation of human bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction

Project description:Differentiation of bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction; reference d-1 (one day before induction; preconfluent 70-80%)

2007-06-09 | E-MARS-3 | biostudies-arrayexpress

Transcription profiling of mouse small intestine intraepithelial lymphocytes (IEL) vs. TCR ab+ CD8b+ and TCR gd+ cells

Project description:Mouse small intestine intraepithelial lymphocytes (IEL) that express a ab TCR and CD8aa homodimers are an enigmatic T cell subset, as their specificity and in vivo function remain to be defined. To gain insight into the nature of these cells, we performed global gene expression profiling using microarray analysis, combined with PCR and flow cytometry to determine the level of expression of selected genes. Using these methods, TCR ab+ CD8aa IEL were compared to their TCR ab+ CD8b+ and TCR gd+ counterparts. Experiment Overall Design: In this study, the three IEL populations were isolated by cell sorting from a pool of IELs from 20 mice. RNA from each IEL subset was used to probe one microarray. A replicate experiment was performed on a seperate occasion using a new pool of 20 mice and three additional microarrays were used.

2008-06-13 | E-GEOD-5355 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data