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The Effects of Regular Post-Exercise Cold Water Immersion on DNA methylation in Muscle Following 10-weeks of Strength Training

ABSTRACT: In this study, the primary objective was to characterise the impact of regular post-exercise (20 strength training sessions across 10 weeks) cold-water immersion (CWI) on DNA methhylation. Secondary to this, the effect of regular post-exercise CWI on strength gains and post-exercise soreness was investigated. We used microarrays to detail the global effects of CWI on DNA methylation in vastus lateralis muscle tissue.

ORGANISM(S): Homo sapiens

PROVIDER: GSE85621 | GEO | 2016/08/16

SECONDARY ACCESSION(S): PRJNA338979

REPOSITORIES: GEO

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The Effects of Regular Post-Exercise Cold Water Immersion on Gene Expression in Muscle Following 10-weeks of Strength Training

Project description:In this study, the primary objective was to characterise the impact of regular post-exercise (20 strength training sessions across 10 weeks) cold-water immersion (CWI) on mRNA expression. Secondary to this, the effect of regular post-exercise CWI on strength gains and post-exercise soreness was investigated. We used microarrays to detail the global effects of CWI on gene expression in vastus lateralis muscle tissue.

2016-08-16 | GSE85620 | GEO

The Effects of Regular Post-Exercise Cold Water Immersion in Muscle Following 10-weeks of Strength Training

Project description:The Effects of Regular Post-Exercise Cold Water Immersion in Muscle Following 10-weeks of Strength Training

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Remodeling of the human skeletal muscle proteome found after long-term endurance training but not after strength training

Project description:Proteomic characterization of skeletal muscle biopsies collected from the vastus lateralis of 44 male and female human subjects. Research subjects were divided in three different groups based on their individual exercise backgrounds and physical performance testing: 1) endurance trained (males; ME, n = 9 and females; FE, n = 9, with at least 15 years’ of regular training experience), 2) strength-trained males (MS, n = 9, with at least 15 years’ of regular training experience), and 3) age-matched healthy untrained controls (males, MC, n = 9 and females FC, n = 8, with a self-reported history of <2 exercise bouts per week over the past 15 years).

2024-01-26 | PXD044445 | Pride

Homo sapiens

Project description:The Effects of Regular Post-Exercise Cold Water Immersion on Gene Expression in Muscle Following 10-weeks of Strength Training

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Homo sapiens

Project description:The Effects of Regular Post-Exercise Cold Water Immersion on DNA methylation in Muscle Following 10-weeks of Strength Training

| PRJNA338979 | ENA

Strength training with suppressed testosterone level

Project description:Human subjects were randomized for treatment with a GnRH-analogue, Goserelin, which suppresses endogenous testosterone or placebo for 12 weeks. Strength training was performed during the last 8 weeks. The suppression of testosterone resulted in an attenuation of the normal muscle adaptation to strength training (increased muscle mass and strength). To identify molecular signals involved in the response to testosterone levels, biopsies were obtained 4 hours after the last training session and gene expression compared with Affymetrix 3' microarrays. This timepoint should capture goserelin effect on both constitutive expression, training induced changes as well as acute exercise induced (4 hours) differences in mRNA levels. Four subject from the placebo group and five subjects from the Goserelin group were compared. Total RNA from vastus lateralis mmuscle biopsies were purified and analyzed on Affymetrix HG-U133 Plus 2.0 arrays.

2014-09-30 | E-GEOD-43437 | biostudies-arrayexpress

Strength training with suppressed testosterone level

2014-09-30 | GSE43437 | GEO

Regular physical activity followed by detraining increases the sensitivity of global gene expression to acute exercise stress

Project description:To determine the influence of a previous physcial activity period (e.g., exercise) on global gene expression, we compared differential expression in sedentary (SED) and detrained (DETR) rats relative to trained (TR) rats with regular access to physcial activity. TR and DETR rats had voluntary access to caged running wheels for 8 and 4 weeks, respectively. DETR rats were moved to sedentary conditions (normal cage activity) for another 4 weeks. SED rats experienced 8 weeks of normal caged activity; n=8 per group. At the end of each group's respective 8 weeks, all rats underwent 1 hr of moderate of acute exercise stress (e.g., forced wheel running at a speed of 20 m/min). Soleus muscles were harvested 24h post-running.

2016-04-05 | E-GEOD-79894 | biostudies-arrayexpress

Regular physical activity followed by detraining increases the sensitivity of global gene expression to acute exercise stress

2016-04-05 | GSE79894 | GEO

Gene expression data from 16-month and 3-month old male rats, with three different levels of activity over the life span: sedentary, twice weekly physical activity, regular running wheel exercise.

Project description:Gene expression data at rest and immediately post 30 min submaximal exercise in 3 month and 16 month old male rats, some of which were sedentary (SED), had access to a physical activity box twice weekly (PA) and some of which had daily access to a running wheel (EX). We used microarrays to detail global gene expression underlying select health-related phenotypes and identified differentially expressed genes among the three groups. Young (3 month) and old (16 month) male rats were separated into three different groups: Sedentary (SED), twice-weekly physical activity (PA), and regular exercise (EX). Animals were sacrificed either at rest or after 30 min of sub maximal exercise. Then, a portion of cardiac tissue was collected for RNA extraction and hybridization on Affymetrix microarrays.

2013-10-18 | E-GEOD-51380 | biostudies-arrayexpress

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