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Gene expression signatures for miR-26a over-expression and negative control in hela cells

ABSTRACT: To further development of our gene expression approach to microRNA-26a over-expression, we have employed whole mRNA microarray expression profiling as a discovery platform. Hela cells at 70–80% confluence in 6-well plates were transfected using Lipofectamine 2000 (Invitrogen). Negative control mimics or miR-26a mimics (100nM) were transfected in each well. Cell extracts were prepared 48 h after transfection, total RNA was checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).

ORGANISM(S): Homo sapiens

PROVIDER: GSE85684 | GEO | 2016/08/17

SECONDARY ACCESSION(S): PRJNA339059

REPOSITORIES: GEO

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