Project description:The mechanism of mecciRNA degradation remains unknown. To investigate the degradation of mecciRNAs, we performed mitochondrial RNA sequencing and total RNA sequencing of 293T, HeLa, and N2a cells. To investigate the degradation mechanism of mecciRNAs, RIP-seq was conducted in 293T and C. elegans. Small RNA sequencing of mitochondrial sucrose gradient fractions was performed to identify mecciRNA degradation fragments.
Project description:We present here the characterization of the replication timing program in 6 human cell lines : U2OS, RKO, 293T, HeLa, MRC5 and K562
Project description:To investigate the function of NCOR2-013 in T. marneffei-infected THP-1 macrophages, we established NCOR2-013 overexpression THP-1 macrphages. We then performed gene expression profiling analysis using data obtained from RNA-seq of T. marneffei-infected NCOR2-013 overexpression THP-1 macrophages and T. marneffei-infected control cells.