Genomics

Dataset Information

162

Paused RNA polymerase II inhibits new transcriptional initiation [ChIP-nexus]


ABSTRACT: RNA polymerase II (Pol II) pauses downstream of the transcription initiation site before beginning productive elongation. This pause is a key component of metazoan gene expression regulation. Some promoters have a strong disposition for Pol II pausing and often mediate faster, more synchronous changes in expression. This requires multiple rounds of transcription and thus cannot rely solely on pause release. But it is unclear how pausing affects the initiation of new transcripts during consecutive rounds of transcription. Using our recently developed ChIP-nexus method, we find that Pol II pausing inhibits new initiation. We propose that paused Pol II helps prevent new initiation between transcription bursts, which may reduce noise. Overall design: ChIP-nexus was performed against Pol II, TBP, TFIIA, TFIIB, TFIIF, TAF2, XPB and NELF-E in Drosophila melanogaster Kc167 cells.

INSTRUMENT(S): Illumina HiSeq 2500 (Drosophila melanogaster)

SUBMITTER: Julia Zeitlinger  

PROVIDER: GSE85738 | GEO | 2017-04-17

SECONDARY ACCESSION(S): PRJNA339233

REPOSITORIES: GEO

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Publications

Paused RNA polymerase II inhibits new transcriptional initiation.

Shao Wanqing W   Zeitlinger Julia J  

Nature genetics 20170515 7


RNA polymerase II (Pol II) pauses downstream of the transcription initiation site before beginning productive elongation. This pause is a key component of metazoan gene expression regulation. Some promoters have a strong disposition for Pol II pausing and often mediate faster, more synchronous changes in expression. This requires multiple rounds of transcription and thus cannot rely solely on pause release. However, it is unclear how pausing affects the initiation of new transcripts during conse  ...[more]

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