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Real-time quantitative PCR analysis of retrogradely labeled stellate neurons in LII of the rat medial entorhinal cortex

ABSTRACT: We compared miRNA expression in FACS-sorted, retrogradely labeled stellate neurons with other medial entorhinal cells, and found 7 miRNAs to be upregulated in stellate cells.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE85752 | GEO | 2017/08/01

SECONDARY ACCESSION(S): PRJNA339269

REPOSITORIES: GEO

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Very deep small RNA sequencing of whole rat medial entorhinal cortex

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Project description:We report the first full transcriptome analysis of layer II and deep layers of the medial and lateral entorhinal cortex during postnatal development. Our analysis showed that postnatal timepoint was the most important element in entorhinal cortex transcriptional dynamics, followed by laminar differences. There were fewer differentially expressed genes between the medial and lateral parts of the entorhinal cortex, and most of these were found in layer II.

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The amyloid precursor protein regulates synaptic transmission at medial perforant path synapses

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2022-09-16 | GSE213284 | GEO

Rattus norvegicus

Project description:Real-time quantitative PCR analysis of retrogradely labeled stellate neurons in LII of the rat medial entorhinal cortex

| PRJNA339269 | ENA

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Project description: Not available

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Stellate Cells in the Medial Entorhinal Cortex Are Required for Spatial Learning.

Project description: Not available

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Project description:Patterns of gene expression in the aged entorhinal cortex and hippocampus were examined one month after entorhinal administration of BDNF lentivirus. Whole-genome patterns of expression were examined using Affymetrix arrays four weeks after entorhinal injection of lentiviral-BDNF or GFP injection compared to control subjects. Experiment Overall Design: 27 Samples total: 4 biological replicates of Age rats BDNF treated, 3 biological replicates of Age rats eGFP treated, and 4 biological replicates each of Aged and Young rats controls for the Entorhinal cortex tissue. 2 biological replicates of Age rats BDNF treated, 2 biological replicates of Age rats eGFP treated, and 4 biological replicates each of Age and Young rats controls for the hippocampus tissue.

2009-01-31 | E-GEOD-14505 | biostudies-arrayexpress

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Project description:All brain functionality arises from the activity in neural circuits in different anatomical regions. These regions contain different circuits comprising unique cell types. An integral part to understanding neural circuits is a full census of the constituent parts, i.e., the neural cell types. This census can be based on different characteristics. Previously combinations of morphology and physiology, gene expression, and chromatin accessibility have been used in various cortical and subcortical regions. This has given an extensive yet incomplete overview of neural cell types. However, these techniques have not been applied to all brain regions. Here we apply single cell analysis of accessible chromatin on two similar but different cortical regions, the medial and the lateral entorhinal cortices. Even though these two regions are anatomically similar, their intrinsic and extrinsic connectivity are different. In 4136 cells we identify 20 different clusters representing different cell types. As expected, excitatory cells show regionally specific clusters, whereas inhibitory neurons are shared between regions. We find that several deep layer excitatory neuronal cell types as defined by chromatin profile are also shared between the two different regions. Integration with a larger scRNA-seq dataset maintains this shared characteristic for cells in Layer Vb. Interestingly, this Layer contains three clusters, two specific to either subregion and one shared between the two. These clusters can be putatively associated with particular functional and anatomical cell types found in this layer. This information is a step forwards into elucidating the cell types within the entorhinal circuit and by extension its functional underpinnings.

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