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Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells [Single Cell RNA-Seq]

ABSTRACT: Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for the clinical application of hPSCs. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrated that the directed switching of defined ECMs robustly yields highly purified (>95%) endothelial progenitor cells (PSC-EPCs) from hPSCs in integrin-laminin axis and rho signaling pathway-dependent manner.

ORGANISM(S): Homo sapiens

PROVIDER: GSE85783 | GEO | 2016/11/08

SECONDARY ACCESSION(S): PRJNA339381

REPOSITORIES: GEO

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Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells [Bulk RNA-Seq]

Project description:Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for the clinical application of hPSCs. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrated that the directed switching of defined ECMs robustly yields highly purified (>95%) endothelial progenitor cells (PSC-EPCs) from hPSCs in integrin-laminin axis and rho signaling pathway-dependent manner.

2016-11-08 | GSE85782 | GEO

Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease (IgG4-RD), a systemic, B-cell driven, fibroinflammatory disorder. To date, numerous autoantigens have been described including laminin 511-E8 and carbonic anhydrase (CA) enzymes (PMID 30089633, 15647194). Laminin 511-E8 is an extracellular matrix protein that has been shown to upregulate secretory components and aid in the cholangiocyte differentiation of induced pluripotent stem cells (PMID 27103433). Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide, thereby producing bicarbonate and a single proton. In humans there are 14 different CA isoforms with a confined subcellular distribution. All isoforms can be targeted by the pan-CA inhibitor acetazolamide, which is a registered drug for multiple indications. We aimed to investigate the role of IgG4-RD autoantigens laminin 511-E8 and CA enzymes in human cholangiocytes. Transcriptional profiling may provide insights into the underlying molecular mechanisms of these proteins on the cholangiocellular level. Thus, by RNA sequencing the transcriptomic signature of recombinant laminin 511-E8 and acetazolamide treatment was assessed in human H69 cholangiocytes.

2024-02-15 | GSE221746 | GEO

Laminin-directed fate determination of human induced pluripotent stem cells (hiPSCs) during ocular cell differentiation

Project description:The extracellular matrix plays a crucial role in stem cell maintenance, expansion and differentiation. Laminin, a basement membrane protein, is effective in supporting cultures of human pluripotent stem cells. To furhter investigate the effects of different isoforms of laminin on ectodemral differentiation of hiPSCs, we performed gene expression analysis of differentiated human iPS cells on E8 fragments of laminin isoforms (LN111E8, LN211E8, LN332E8, LN411E8, and LN511E8).

2018-10-17 | GSE116591 | GEO

Laminin binding/non-binding germ cells

Project description:Comparison of laminin binding and laminin non-binding germ cells Keywords: other

2004-03-16 | GSE829 | GEO

Transcription profiling of laminin binding and laminin non-binding germ cells to define the spermatogonial stem cells.

Project description:Comparison of laminin binding and laminin non-binding germ cells.

2007-12-19 | E-GEOD-829 | biostudies-arrayexpress

Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis

Project description:We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) scaffolds that can be repopulated with various cell types to interrogate cell-ECM interactions. Using this platform, we investigated whether fibrotic ECM scarring affected human skeletal muscle progenitor cell (SMPC) functions that are essential for myoregeneration. SMPCs exhibited robust adhesion, motility, and differentiation on healthy muscle-derived myoscaffolds. All SPMC interactions with fibrotic myoscaffolds from dystrophic muscle were severely blunted including reduced motility rate and migration. Furthermore, SMPCs were unable to remodel laminin dense fibrotic scars within diseased myoscaffolds. Proteomics and structural analysis revealed that excessive collagen deposition alone is not pathological, and can be compensatory, as revealed by overexpression of sarcospan and its associated ECM receptors in dystrophic muscle. Our in vivo data also supported that ECM remodeling is important for SMPC engraftment and that fibrotic scars may represent one barrier to efficient cell therapy.

2022-12-29 | GSE221912 | GEO

miRNA expression profile of mouse Lewis lung carcinoma LLC1 cell line cultivated in 2-dimensional or 3-dimensional cell culture enriched with laminin rich extracellular matrix proteins

Project description:The extracellular matrix (ECM), as one of the key components of tumor microenvironment has a high impact on cancer development and highly influences tumor cell features. ECM contribute not only structural support to growing tumor cells, but also affect other cellular functions such as cell differentiation, migration, survival or proliferation. Moreover, gene and protein expression levels are regulated in cell-ECM interaction dependent manner. MicroRNAs (miRNAs) act as key modulators of gene expression in various basic cellular processes where they can decrease protein synthesis through translational repression or RNA degradation. Despite miRNAs have recently emerged as important players involved in regulation of cell-ECM interactions molecular mechanisms of this phenomenon remains to be elucidated. To address this question we explored miRNA expression patterns in mouse Lewis lung carcinoma LLC1 cells growing in laminin rich ECM microenvironment. Total RNA enriched in small noncoding RNAs was isolated 48h after growing in two different cell culture systems

2015-12-10 | E-GEOD-75862 | biostudies-arrayexpress

Laminin-deficient muscular dystrophy, dy/dy diaphragm

Project description:Laminin (merosin) deficient muscular dystrophy in dy/dy mouse diaphragm muscle, 8 weeks old Keywords: muscle, muscular dystrophy, laminin, merosin, diaphragm, mouse

2006-03-16 | GSE3252 | GEO

Galectin-3 and prohibitin 1 are autoantigens in IgG4-related cholangitis without protective effects against toxic bile acids

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease, a systemic B-cell-driven fibroinflammatory disorder. Four autoantigens have recently been described in IgG4-RD; annexin A11, galectin-3, laminin 511-E8, and prohibitin 1. We have previously reported a protective role of annexin A11 and laminin 511-E8 in human cholangiocytes against toxic bile acids. Here we explored a potentially protective role of the carbohydrate binding lectin galectin-3 and the scaffold proteins prohibitin 1 and 2.

2024-01-23 | PXD042856 | Pride

miRNA expression profile of mouse Lewis lung carcinoma LLC1 cell line cultivated in 2-dimensional or 3-dimensional cell culture enriched with laminin rich extracellular matrix proteins

2015-12-10 | GSE75862 | GEO

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