Genomics

Dataset Information

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Epigenome Mapping in pancreatic cancer cells


ABSTRACT: Genome binding/occupancy profiling of H3K4me1 and H3K4me3 upon KMT2D depletion was performed by Illumina NextSeq500 using single‐end 75bp protocol. Overall design: Chromatin fragments, derived from siControl and siKMT2D (using 2 different siRNAs) treated cells, were immunoprecipitated with antibodies against Anti-Histone H3 (mono-methyl K4) and Anti-Histone H3 (tri-methyl K4). After purification of DNA from cell lysate by functionalized magnetic particles, libraries for next generation sequencing were prepared and analyzed using standard procedures.

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

SUBMITTER: MARINA KOUTSIOUMPA  

PROVIDER: GSE85886 | GEO | 2018-08-20

REPOSITORIES: GEO

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Publications


<h4>Objective</h4>Despite advances in the identification of epigenetic alterations in pancreatic cancer, their biological roles in the pathobiology of this dismal neoplasm remain elusive. Here, we aimed to characterise the functional significance of histone lysine methyltransferases (KMTs) and demethylases (KDMs) in pancreatic tumourigenesis.<h4>Design</h4>DNA methylation sequencing and gene expression microarrays were employed to investigate CpG methylation and expression patterns of KMTs and K  ...[more]

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