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Dosage compensation in the mouse balances up regulation and silencing of X-linked genes

ABSTRACT: Dosage compensation in mammals involves silencing of one X chromosome in XX females, and requires expression, in cis, of Xist silencing RNA. Using microarray analysis to assay expression of X-linked genes in mouse embryonic stem cells, we show that dosage compensation also involves global up-regulation of expression from the active X in both males and females. Up-regulation is complete (ie. 2-fold) only after 2-3 weeks of differentiation. X-linked gene silencing in female cells occurs on a gene-by-gene basis throughout differentiation and includes a sub-group of genes silenced prior to the onset of differentiation and independently of enhanced Xist expression. Keywords: dosage compensation, gene silencing, Xist RNA, microarray

ORGANISM(S): Mus musculus

PROVIDER: GSE8593 | GEO | 2007/12/01

SECONDARY ACCESSION(S): PRJNA101751

REPOSITORIES: GEO

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Transcription profiling of mouse embryonic stem cells during differentiation to investigate dosage compensation by assaying expression of X-linked genes

Project description:Dosage compensation in mammals involves silencing of one X chromosome in XX females, and requires expression, in cis, of Xist silencing RNA. Using microarray analysis to assay expression of X-linked genes in mouse embryonic stem cells, we show that dosage compensation also involves global up-regulation of expression from the active X in both males and females. Up-regulation is complete (ie. 2-fold) only after 2-3 weeks of differentiation. X-linked gene silencing in female cells occurs on a gene-by-gene basis throughout differentiation and includes a sub-group of genes silenced prior to the onset of differentiation and independently of enhanced Xist expression. Differentiation of embryonic stem cells was induced by the removal of leukemia inhibitory factor (LIF) from the media and time values refer to time since LIF removal.

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