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RNA-seq of KD, rescues of NMD factors, and UPF1-flag CLIP-seq in HeLa cells.

ABSTRACT: This study aims at confidently identifying endogenous nonsense mediated decay (NMD) targets. To achieve this purpose, we performed KD of a few NMD factors in HeLa cells. Additionally, we performed rescue experiments for each factor, expressing an RNAi-resistant version of the gene from a plasmid. To determine transcripts bound by UPF1 in HeLa cells, A construct with a C-terminally flag tagged version of UPF1 was expressed. In order to avoid competition with endogenous UPF1, a KD was performed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE86148 | GEO | 2016/12/19

SECONDARY ACCESSION(S): PRJNA340370

REPOSITORIES: GEO

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