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Yeast mRNA decay analysis after addition of 3 & 10 µ/mL thiolutin

ABSTRACT: Time course after the addition of the transcriptional inhibitor thiolutin at 3 and 10 µg/mL to an exponential growing culture of S. cerevisiae in YPD. Keywords: time course, mRNA stability analysis

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE8629 | GEO | 2007/11/29

SECONDARY ACCESSION(S): PRJNA101811

REPOSITORIES: GEO

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Yeast mRNA decay analysis after addition of 3 µ/mL thiolutin

Project description:Time course after the addition of the transcriptional inhibitor thiolutin at 3µg/mL to an exponential growing culture of S. cerevisiae in YPD. Keywords: time course, mRNA stability analysis

2007-11-17 | GSE7261 | GEO

Yeast mRNA decay analysis after addition of 3 & 10 Âµ/mL thiolutin

Project description:Time course after the addition of the transcriptional inhibitor thiolutin at 3 and 10 Âµg/mL to an exponential growing culture of S. cerevisiae in YPD. Total RNA from BQS252 yeast strain (Mat a, ura3-52 derived from FY1679) growing in exponential phase in YPD was extracted at 30 minutes after thiolutin 3 and 10 Âµg/mL addition. All the samples were hybridized on the same nylon array. 2 replicates.

2010-06-25 | E-GEOD-8629 | biostudies-arrayexpress

Yeast mRNA decay analysis after addition of 3 Âµ/mL thiolutin

Project description:Time course after the addition of the transcriptional inhibitor thiolutin at 3Âµg/mL to an exponential growing culture of S. cerevisiae in YPD. Total RNA from BQS252 yeast strain (Mat a, ura 3-52 derived from FY1679) growing in exponential phase in YPD was extracted at different times after thiolutin 3Âµg/mL addition. All the samples were hybridized on the same nylon array. In order to estimate mRNA stability the intensities where normalized using global poly(A) data from a RNA dot-blot and assuming a first order decaying. There is only 1 replicate.

2010-06-25 | E-GEOD-7261 | biostudies-arrayexpress

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Impact of Nonsense-mediated mRNA Decay on the Global Expression Profile of Budding Yeast

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