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Omics score: 28

VLX60 characterization II

ABSTRACT: Microarray based mRNA profiling was used to identify the mechanism of action for the small molecule VLX60. Overall design: HCT116 cells were treated with the drug candidate VLX50 or VLX60 for 6h prior to RNA isolation

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Mårten Fryknäs

PROVIDER: GSE86309 | GEO | 2017-07-04

SECONDARY ACCESSION(S): PRJNA341386

REPOSITORIES: GEO

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Publications

Mechanistic characterization of a copper containing thiosemicarbazone with potent antitumor activity.

Karlsson Henning H Fryknäs Mårten M Strese Sara S Gullbo Joachim J Westman Gunnar G Bremberg Ulf U Sjöblom Tobias T Pandzic Tatjana T Larsson Rolf R Nygren Peter P

Oncotarget 20170501 18

<h4>Background</h4>The thiosemicarbazone CD 02750 (VLX50) was recently reported as a hit compound in a phenotype-based drug screen in primary cultures of patient tumor cells. We synthesized a copper complex of VLX50, denoted VLX60, and characterized its antitumor and mechanistic properties.<h4>Materials and methods</h4>The cytotoxic effects and mechanistic properties of VLX60 were investigated in monolayer cultures of multiple human cell lines, in tumor cells from patients, in a 3-D spheroid cel ...[more]

PMID: 28415818

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