Genomics

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Transcriptomic assessment of PCB-resistance in Atlantic killifish (Fundulus heteroclitus) embryos from a marine Superfund site


ABSTRACT: RNA from PCB-126 exposed 10 days post-fertilization (dpf) embryos were sequenced to characterize the chemical exposure response of killifish from a PCB-resistant population (NBH, New Bedford Harbor, MA, Superfund site) as compared to a reference (SC, Scorton Creek, MA) population. Since the genome sequence was not available, we also performed shot-gun sequencing of RNA from 1-15 dpf embryos sampled every day from both populations to serve as a transcriptome assembly. The results suggest that the NBH fish possess a gene regulatory defect that is not limited to a few target genes. We detected multiple genes that were differentially expressed in these two populations. This study was the first application of pyrosequencing technology to combine transcriptome characterization and gene expression profiling in a marine animal.

ORGANISM(S): Fundulus heteroclitus

PROVIDER: GSE86593 | GEO | 2016/09/09

SECONDARY ACCESSION(S): PRJNA342319

REPOSITORIES: GEO

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