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Heat shock experimental replicates

ABSTRACT: Heat stress experiments imposed on Buchnera aphidicola within intact Schizaphis graminum hosts. NOTE: normalized values in GEO are NOT those derived from intensive analyses described in paper. Please see Wilcox et al. 2002 for description. Keywords = reduced bacterial genome, stress response Keywords: repeat sample

ORGANISM(S): Buchnera aphidicola (Schizaphis graminum)

PROVIDER: GSE87 | GEO | 2003/01/21

SECONDARY ACCESSION(S): PRJNA85467

REPOSITORIES: GEO

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Heat shock experimental replicates

Project description:Heat stress experiments imposed on Buchnera aphidicola within intact Schizaphis graminum hosts. NOTE: normalized values in GEO are NOT those derived from intensive analyses described in paper. Please see Wilcox et al. 2002 for description. Keywords = reduced bacterial genome, stress response

2003-01-20 | E-GEOD-87 | biostudies-arrayexpress

Responses of the Rhodobacter sphaeroides Transcriptome to Blue Light

Project description:Data used in "Responses of the Rhodobacter sphaeroides Transcriptome to Blue Light Under Semiaerobic Conditions" paper. Cells are grown at 10% O2 (see detailed description in the paper and in the sample info). Keywords: time-course

2004-06-17 | GSE1480 | GEO

Identifying Significant Temporal Variation in Time Course Microarray Data Without Replicates

Project description:An important component of time course microarray studies is the identification of genes that demonstrate significant time-dependent variation in their expression levels. Until recently available methods for performing such significance tests required replicates of individual time points. This paper describes a replicate-free method that was developed as part of a study of the estrous cycle in the rat mammary gland in which no replicate data was collected. Keywords: developmental time course (time series) throughout the estrus cycle in rat mammary gland singlet data

2008-08-04 | GSE12289 | GEO

The YmdB phosphodiesterase is a global regulator of biofilm-specific mRNA levels in Bacillus subtilis

Project description:Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper.

2013-12-11 | GSE39142 | GEO

Expression data from rice under salinity stress

Project description:Expression Data of Rice Crown and Growing Point Tissue Under Salt Stress imposed during the Panicle Initiation Stage Keywords: genotypes and growth conditions (control and salt stressed)

2006-09-15 | GSE4438 | GEO

Expression data from three rice lines (1-control, 1-transgenic and 1-negative segregant) throughout generations and under salt stress

Project description:The approval of genetically modified (GM) crops is preceded by years of intensive research to demonstrate safety to humans and environment. We recently showed that in vitro culture stress is the major factor influencing proteomic differences of GM vs. non-GM plants. This made us question the number of generations needed to erase such memory. We also wondered about the relevance of alterations promoted by transgenesis as compared to environment-induced ones. Here we followed three rice lines (1-control- C, 1-transgenic- Ta and 1-negative segregant- NSb) throughout eight generations after transgenesis, and further analyzed their response to salinity stress on the F6 generation. Three pools of 10 whole fifteen days-old rice seedlings (Oryza sativa L. ssp. japonica cv. Nipponbare) were selected from each line at F4, F6 and F8 generations. Because salinity stress was imposed on half of the seedlings (C, Ta and NSb) in F6 generation, from this generation onwards we worked with six rice lines (C, Csalt, Ta, Tasalt, NSb, NSbsalt).

2016-08-03 | E-GEOD-85113 | biostudies-arrayexpress

Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase

Project description:Comparison of Bacillus subtilis wild type and cshA mutant at exponential versus stationary phase. Detailed description (other than provided below) of growth conditions, RNA preparation, cDNA synthesis and hybridization conditions can be found in the submitted paper.

2012-11-26 | GSE36877 | GEO

Comparison of normal and heat shock trophozoites

2007-03-01 | GSE6650 | GEO

Comparison of normal and heat shock trophozoites

Project description:We examined the stress response in Entamoeba histolytica trophozoites by comparing untreated log-phase HM-1:IMSS trophozoites to those subjected to heat shock at 42C for 1 hour. Keywords: stress reponse We compared two arrays from normal trophozoites to two arrays from trophozoites subjected to heat shock.

2007-03-01 | E-GEOD-6650 | biostudies-arrayexpress

Effect of heat shock on Rickettsia prowazekii Madrid E from four independent biological replicates

Project description:Here we present the first microarray analysis of global gene expression changes in the obligate intracytoplasmic pathogen Rickettsia prowazekii using temperature upshift as a model stress condition. We describe a methodology for isolating highly-purified rickettsial RNA where a combination of differential centrifugation and use of Ambion’s MICROBEnrich reagent removed the majority (~90 %) of host cell RNA. A pool of rickettsiae-infected L-cells grown at 34 oC was split and half of the sample was challenged at 42 oC for 30 minutes, total RNA was isolated and converted to cDNA followed by hybridization to an oligonucleotide DNA array. We report data (duplicate hybridizations) from four, independent biological replicates. Twenty-three rickettsial transcripts were significantly increased by temperature upshift greater than two-fold and no transcripts demonstrated reproducible decreases. Notably, the GroESL, DnaK, and DnaJ members of the family of chaperones as well as the two R. prowazekii proteins annotated as putative heat shock proteins were induced. In addition, proteins annotated as proteases were also induced indicating that R. prowazekii mounts a response to heat-shock induced protein unfolding. These data are the first global analysis of the R. prowazekii transcriptome and provide insight into the mechanisms employed by rickettsiae to adapt to temperature upshifts.

2008-08-08 | GSE12377 | GEO

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