Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in Wip1+/+ or -/- MEFs after gamma radiation

ABSTRACT: Assessment of p53 targets by gene expression array analysis in irradiated and nonirradiated Wip1+/+ and Wip1-/- MEFs. Keywords: response to gamma radiation

ORGANISM(S): Mus musculus

PROVIDER: GSE8704 | GEO | 2007/08/08

SECONDARY ACCESSION(S): PRJNA101937

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of mouse embryonic fibroblasys from Wip1+/+ or -/- animals after gamma radiation

Project description:Assessment of p53 targets by gene expression array analysis in irradiated and nonirradiated Wip1+/+ and Wip1-/- MEFs. Experiment Overall Design: mRNA from each set of cells harvested four hours after IR (5 Gy) was Experiment Overall Design: used for gene expression microarray analysis of p53 target genes using the Affymetrix Mouse Genome 430 2.0 Chips.

2008-06-16 | E-GEOD-8704 | biostudies-arrayexpress

Microarray analysis of gene expression in ATM-deficient MEFs

Project description:The expression of interferon-related genes was more enhanced in irradiated ATM-deficient mouse embryonic fibroblasts (MEFs) than in irradiated ATM wild-type MEFs. Nonirradiated-ATM-WT vs Irradiated-ATM-WT vs Nonirradaited-ATM-KO vs IrradiatedATM-KO

2012-06-19 | E-GEOD-23116 | biostudies-arrayexpress

Microarray analysis of gene expression in mouse embryonic fibroblasts (MEFs) by addition of serum from L/MDR irradiated mice at 10 and 20 days.

Project description:The expression changes were detected by addition of serum form L/MDR irradiated mice at 10 and 20 days in mouse embryonic fibroblasts (MEFs). (Expression of MEFs by addition of serum from nonirradiated mice) vs (Expression of MEFs by addition from L/MDR irradiated mice)

2013-12-31 | E-GEOD-37958 | biostudies-arrayexpress

Expression data from murine embryonic fibroblast with/without radiation.

Project description:To identify the STING-dependent genes that play a role in facilitating the immunogenicity of DNA-damaged cells, normal MEFs or MEFs locking STING, cGAS, or IRF3 were irradiated. We used microarrays to detail the global programme of gene expression underlying the upregulation of several STING-induced genes in response to radiation.

2024-09-01 | GSE270238 | GEO

DIA-based quantitative proteomics of Staphylococcus aureus exposed to gamma radiation

Project description:S. aureus was exposed to gamma radiation at an irradiation dose of 100 Gy, and its descendant were cultured under normal conditions. Protein identification and quantification of unirradiated, irradiated, and descendant of irradiated S. aureus were performed using DIA proteomic technology to obtain protein abundance profiles for each group. Combined with biological experiments, further functional enrichment analysis and protein-protein interaction network analysis were performed to explore the effect of gamma radiation on the pathogenicity of S. aureus.

2024-07-20 | PXD054090 |

Genome-wide microarray analysis of normal human fibroblasts in response to γ-radiation and the radiation induced bystander effect

Project description:The bystander effect from ionizing radiation consists of cellular responses generated from non-irradiated cells to the irradiation of their neighbors. The bystander effect is predominant at low doses and can lead to DNA damage and genomic instability in the affected cells. This non-targeted effect of radiation has received attention due to its potential implications for cancer therapy and radiation protection. Although studied extensively, a complete understanding of its molecular mechanism is the subject of ongoing research. While many studies have targeted specific factors which are suggested to be involved in the bystander effect, few have looked at whole genome gene expression in bystander cells. Furthermore, even fewer studies have looked at the expression in normal human cell lines. In this study, we have monitored transcriptional responses to γ-radiation in irradiated and bystander normal fibroblasts simultaneously using a genome-wide microarray approach. Bystander fibroblasts incubated in medium from irradiated cells, showed transient enrichment (less than 1.5 fold) in ribosome and oxidative phosphorylation pathways, and neurodegenerative disease pathways associated with mitochondrial dysfunctions. Bystander fibroblasts did not, however, display increases in oxidative stress, a phenomenon often linked with the radiation induced bystander effect. Total RNA was isolated from normal human fibroblasts irradiated with 2.0 Gy and fibroblasts incubated with medium from sham irradiated and irradiated cells 2 h after irradiation. RNA was isolated 4, 8 and 26 h after irradiation and there are 4 replicates for each sample for a total of 36 samples.

2010-12-03 | E-GEOD-25772 | biostudies-arrayexpress

Microarray analysis of gene expression in ATM-deficient MEFs

Project description:The expression of interferon-related genes was more enhanced in irradiated ATM-deficient mouse embryonic fibroblasts (MEFs) than in irradiated ATM wild-type MEFs.

2012-06-20 | GSE23116 | GEO

Genome-wide microarray analysis of normal human fibroblasts in response to γ-radiation and the radiation induced bystander effect

2010-12-03 | GSE25772 | GEO

Transcriptome analysis of Zeugodacus cucurbitae in response to 60Cobalt gamma radiation.

Project description:The goal of the current study is to know the possible molecular mechanism of the effects of 60Co radiation stress when eggs of Z. cucurbitae were exposed to the radiation dose of 50 Gy. A comparative transcriptome analysis was performed to investigate the global gene expression changes in 3rd instar of Z. cucurbitae emerged from the irradiated eggs. Hence we described the Transcriptome of Z. cucurbitae using Illumina Hiseq 4000 platform. To identify the DEGs between irradiated and non-irradiated flies, we performed a DGE (Digital Gene Expression) analysis in the non-irradiated group and compared this analysis with flies after the irradiation. This approach was a shortcut to identify the genes involved in the irradiation-induced developmental defects and growth regulation in Z. cucurbitae. Based on searches against databases, deferentially expressed genes (DEGs) were classified and enriched in different functional categories and pathways.

2023-01-13 | GSE194002 | GEO

Wip1 regulates adult neurogenesis and Wnt signaling during aging

Project description:The number of newly-formed neurons declines rapidly during aging. Here we describe an important mechanism that contributes to this decline via Wip1-dependent regulation of neuronal differentiation. We found that Wip1 is expressed in neural stem/progenitor cells (NPCs) of the mouse subventricular zone and its upregulation at physiological levels maintained higher NPC numbers and neuronal differentiation in old mice. This resulted in markedly improved neuron formation and rescued a functional defect in fine odor discrimination in old mice. We identified Dkk3 as a key downstream target of Wip1 and found that its expression in SVZ is restricted to NPCs. Functionally, Dkk3 inhibited neuroblast formation by suppressing Wnt signaling, while deletion of Dkk3 or pharmacological reactivation of the Wnt pathway improved neuron formation and olfactory function in aged mice. We propose that Wip1 controls a Dkk3-dependent inhibition of neuronal differentiation during aging and thus regulating Wip1 levels could prevent certain aspects of functional decline of the aging brain. We found if neurospheres were derived from 18 months old mice, Wip1 transgenic neurospheres were more neurogenic than wt ones. This microarray was a pilot experiment to search the mechanism how Wip1 Transgene promoted neurogenesis, and found Dkk3 as a potential mediator.

2014-04-26 | GSE57105 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data