Dataset Information


Genes encoding cognate receptors for IRI-related recipient cytokines are expressed in donor livers

ABSTRACT: Purpose: RNAseq was used to look in donor allografts for genes encoding cytokine receptors specific to the cytokines identified to be upregulated in the blood of Orthotopic liver transplantation (OLT) receipients with ischemia reperfusion injury (IRI) Methods: Liver mRNA cytokine receptor profiles of allografts taken before and after transplant, demultiplexed with Illumina CASAVA, trimmed with Cutadapt, aligned to hg38 with STAR, counted with featureCounts Results: We identified expression of genes encoding 10 cognate receptors for the 14 potential cytokines found to be upregulated in IRI+ patients. Conclusion: Many of the cytokines that are upregulated in the blood of IRI+ OLT recipients have cognate receptors present in donor livers that could allow completed signaling pathways to occur Overall design: Liver allograft mRNA profiles of pre- and post-reperfusion biopsies from IRI+ and IRI- OLT recipients were generated by deep sequencing using Illumina HiSeq 2000

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

ORGANISM(S): Homo Sapiens

SUBMITTER: Rebecca A. Sosa  

PROVIDER: GSE87487 | GEO | 2016-12-08



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<b>BACKGROUND.</b> Orthotopic liver transplant (OLT) is the primary therapy for end-stage liver disease and acute liver failure. However, ischemia/reperfusion injury (IRI) can severely compromise allograft survival. To understand the evolution of immune responses underlying OLT-IRI, we evaluated longitudinal cytokine expression profiles from adult OLT recipients before transplant through 1 month after transplant. <b>METHODS.</b> We measured the expression of 38 cytokines, chemokines, and growth  ...[more]

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