Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression data from mice Ago2-amygdala

ABSTRACT: In this study we aimed to uncover which mRNAs are changed in the Ago2-amygdala complex following chronic social defeat in mice We used microarrays to determine which mRNAs are changed in the Ago2-amygdala complexof mice following chronic social defeat compared to control

ORGANISM(S): Mus musculus

PROVIDER: GSE87489 | GEO | 2016/09/30

SECONDARY ACCESSION(S): PRJNA344900

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

miRNA expression data from mice Ago2-amygdala

Project description:In this study we aimed to uncover which miRNAs are changed in the Ago2-amygdala complex following chronic social defeat in mice We used microarrays to determine which miRNAs are changed in the Ago2-amygdala complexof mice following chronic social defeat compared to control

2016-09-30 | GSE87488 | GEO

Gene expression analysis of mouse BNST and blood cells after chronic social defeat stress (CSDS)

Project description:Chronic psychosocial stress is a risk factor for psychiatric disorders, and genetic factors interact in conferring susceptibility. The chronic social defeat stress (CSDS) mouse model allows identifying factors underlying resilience and susceptibility to chronic psychosocial stress. Following 10 days of CSDS, we collected the brain and blood tissues. We performed (1) AGO2 RNA immunoprecipitation-sequencing (AGO2 RIP-seq) of active miRNAs and their mRNA targets in the BNST. Additionally, we carried out RNA-seq and miRNA-seq from blood cells.

2019-08-15 | GSE122840 | GEO

Rat, male, brain, medial amygdala, LC-MSMS

Project description:Social isolation poses a severe mental and physiological burden on humans. Most animal models that investigate this effect are based on prolonged isolation, which does not mimic the milder conditions experienced by people in the real world. Here we show that in the medial amygdala, a brain structure that is crucial for social memory, acute social isolation causes social memory loss and significant changes in specific mRNAs and proteins.

2021-11-03 | PXD026871 | Pride

Small RNA Sequencing of mouse amygdala after knockdown of miR-15a

Project description:MicroRNAs are important regulators of gene expression and associated with stress-related psychiatric disorders. We report that exposing mice to chronic stress led to a specific increase in microRNA-15a levels in the amygdala-Ago2 complex, and a concomitant reduction in the levels of its predicted target, FKBP51, which is implicated in stress-related psychiatric disorders. Reciprocally, mice expressing reduced levels of amygdalar microRNA-15a following exposure to chronic stress exhibited increased anxiety-like behaviors. Here, we performed small RNA Sequencing of mouse basolateral amygdala after miR15a knockdown using injection of a miR-15a sponge virus or control sponge virus.

2016-10-01 | GSE87533 | GEO

mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

Project description:As a core RISC component, Ago2 associates with miRNAs and target mRNAs. To identify these mRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2, +mock transfection. To identify mRNAs associated with specific miRNAs, we ran lysate from HEK293T cells over a FLAG resin from 2 conditions: +FLAG-Ago2 & miR-1, and +FLAG-Ago2 & miR-124. Set of arrays that are part of repeated experiments Compound Based Treatment: mock transfected Keywords: Biological Replicate

2008-04-08 | GSE11079 | GEO

Proteomic and transcriptional profiling of rat amygdala following social play

Project description:Social play was performed on one cohort of adolescent rats. Other cohort was remaining naïve to behavioral testing. The rats were scarified following behavioral testing on postnatal day 38. Amygdala was isolated form the brain, homogenized the tissue, proteins were quantified, trypsin digested, and analyzed by LC ESI MS/MS.

2019-11-06 | PXD012677 | Pride

mRNAs associated with Ago2: Ago2, Ago2+miR-1, Ago2+miR-124, mock transfected

2008-04-08 | E-GEOD-11079 | biostudies-arrayexpress

RNA sequencing approaches to identify the differential expression gene in the mPFC with AAV-mediated Npas4 shRNA expression

Project description:Chronic social defeat stress induces anhedonia-like behavior deficits. We demonstrated that mPFC NPAS is requred for chronic social defeat stress-induced deficits in the sucrose preference and effort-based reward seeking behavior. We conducted the RNA-seq analysis for differential expression genes in the mouse mPFC samples of AAV-mediated Npas4 shRNA expression tissue.

2023-02-10 | GSE165586 | GEO

BAZ1B in Nucleus Accumbens Regulates Reward-Related Behaviors in Response to Distinct Emotional Stimuli

Project description:ATP-dependent chromatin remodeling proteins are being implicated increasingly in the regulation of complex behaviors, including models of several psychiatric disorders. Here, we demonstrate that Baz1b, an accessory subunit of the ISWI family of chromatin remodeling complexes, is upregulated in the nucleus accumbens (NAc), a key brain reward region, in both chronic cocaine-treated mice and mice that are resilient to chronic social defeat stress. In contrast, no regulation is seen in mice that are susceptible to this chronic stress. Viral-mediated overexpression of Baz1b, along with its associated subunit Smarca5, in mouse NAc is sufficient to potentiate both rewarding responses to cocaine, including cocaine self-administration, and resilience to chronic social defeat stress. However, despite these similar, proreward behavioral effects, genome-wide mapping of BAZ1B in NAc revealed mostly distinct subsets of genes regulated by these chromatin remodeling proteins after chronic exposure to either cocaine or social stress. Together, these findings suggest important roles for BAZ1B and its associated chromatin remodeling complexes in NAc in the regulation of reward behaviors to distinct emotional stimuli and highlight the stimulus-specific nature of the actions of these regulatory proteins. BAZ1B (WSTF) ChIP-seq of mouse. Cocaine vs Saline, 3 biological replicates. In social defeat model: Normal control vs Susceptible vs Resilient, 3 biological replicates.

2016-07-30 | E-GEOD-85005 | biostudies-arrayexpress

Proteomic and transcriptional profiling of rat amygdala following social play

Project description:Proteomic and transcriptional profiling of rat amygdala following social play

| PRJNA518911 | ENA