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Next generation sequencing facilitates transcriptome analyses of mock and RHCG transfected esophageal squamous cell carcinoma cell line KYSE30 cells

ABSTRACT: We obtained transcriptome profiling (RNA-seq) of human esophageal squamous cell carcinoma cell line KYSE30 cells stabley transfected with pIRES2-EGFP vector or human RHCG-expression vector by using next generation sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE87664 | GEO | 2019/09/26

REPOSITORIES: GEO

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Next generation sequencing facilitates transcriptome analyses of mock and NCCRP1 transfected esophageal squamous cell carcinoma cell line KYSE150 cells

Project description:We obtained transcriptome profiling (RNA-seq) of human esophageal squamous cell carcinoma cell line KYSE150 stabley transfected clones with pIRES2-EGFP vector or human NCCRP1-expression vector by using next generation sequencing.

2019-09-26 | GSE87665 | GEO

Expression analysis of gene expression chang in Homo sapiens Esophageal squamous cell carcinoma cells KYSE30 after knock down MTA2 gene

Project description:Investigation of whole genome gene expression level changes in Homo sapiens Esophageal squamous cell carcinoma cells KYSE30 after knock down of MTA2 gene expression

2021-03-10 | GSE112495 | GEO

A Long Noncoding RNA Acts as a Tumor Suppressor in Esophageal Squamous Cell Carcinoma

Project description:Many long noncoding transcripts are involved in cancer progression. Here, we utilized high-throughput microarray to compare the transcriptome alterations between the KYSE30 cells overexpressing an empty vector and Epist, thus identified 95 and 99 down-regulated and up-regulated genes, respectively. Expression levels of several previously reported genes implicated in cancer development and progression were altered, such as DDIT3, GATA6, UPP1, FAT3. These genes are involved in tumorigenesis through diverse mechanisms. The present study reveals that Epist functions as a tumor suppressor in Esophageal Squamous Cell Carcinoma. KYSE30 cells overexpressing an empty vector were compared to KYSE30 cells overexpressing Epist. Three biological replicates of each condition were analysed on Agilent microarray.

2015-01-09 | E-GEOD-64792 | biostudies-arrayexpress

Human Esophageal Squamous Cell Cancer Cell line: Control vs. mir203 expression vector Transfected

Project description:Transcriptional profiling of Human Esophageal Squamous Cell Cancer Cell line (KYSE520) comparing mock transfectant (KYSE520 Mock②) with cells transfected with a mir203 expression vector (KYSE520 miR203⑥)

2014-08-22 | GSE50615 | GEO

A Long Noncoding RNA Acts as a Tumor Suppressor in Esophageal Squamous Cell Carcinoma

2015-01-09 | GSE64792 | GEO

Human Esophageal Squamous Cell Cancer Cell line: Control vs. mir203 expression vector Transfected

2014-08-22 | E-GEOD-50615 | biostudies-arrayexpress

MicroRNAs among esophageal cancer cell sublines with different migration ability

Project description:Cellular directed migration is critical to invasion-metastasis cascade of cancer cells. We used in vitro transwell model to screen two esophageal cancer cell lines (KYSE30/180) and obtained two pairs of subpopulations with distinc motiliyt ability. Then we used microarrays to detail the differentially expressed genes and microRNAs between these two cell sublines (30U/D and 180U/D) to identify those responsible for ESCC motility. KYSE30/180 cells were subject to four successive selections using transwell (CORNING, USA). Subpopulations penetrated through membrane (D) or not (U) were harvested respectively for RNA extraction and hybridization on Affymetrix genome and LC Sciences microRNA microarrays

2015-12-31 | E-GEOD-67509 | biostudies-arrayexpress

Expression data among esophageal cancer cell sublines with different migration ability

Project description:Cellular directed migration is critical to invasion-metastasis cascade of cancer cells. We used in vitro transwell model to screen two esophageal cancer cell lines (KYSE30/180) and obtained two pairs of subpopulations with distinct motility ability. Then we used microarrays to detail the differentially expressed genes and microRNAs between these two cell sublines (30U/D and 180U/D) to identify those responsible for ESCC motility. KYSE30/180 cells were subject to four successive selections using transwell (CORNING, USA). Subpopulations penetrated through membrane (D) or not (U) were harvested respectively for RNA extraction and hybridization on Affymetrix genome and LC Sciences microRNA microarrays simultaneously.

2015-12-31 | E-GEOD-67508 | biostudies-arrayexpress

Effect of Fusobacterium nucleatum (Fn) infection or Fn-Dps treatment on gene expression of esophageal carcinoma Kyse150 and Kyse30 cells

Project description:Kyse150 and Kyse30 cells were infected by Fn (the MOI of 1:10) or treated with Fn-Dps (1 μM) for 48 h

2023-06-01 | GSE222917 | GEO

Transcriptomes Analysis of LHX2-knockdown in KYSE30 and KYSE510 cells

Project description:Purpose: To fully realize the potential molecular mechanism that LHX2 promotes ESCC progression Methods: Total RNA of LHX2-knockdown KYSE30/KYSE510 and control cells was extracted with TRIzol Reagent. RNA libraries were constructed using an Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol. A total of 150 base paired-end reads were sequenced using the Novaseq 6000 S4 platform. Results: We identified 26008 transcripts in KYSE30 control and KYSE30 LHX2-knockdown cells ,and 25561 transcripts in KYSE510 control and KYSE510 LHX2-knockdown cells. Conclusions: Our study represents the analysis of LHX2-knockdown ESCC cells, generated by RNA-seq.

2023-04-07 | GSE209942 | GEO

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