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Maedi-visna virus (MVV) preferrentially integrates into genes and generates 6-bp duplications

ABSTRACT: We sequenced MVV integration sites in acutely infected primary sheep cells and compared them to a control set of integration sites generated using recombinant MVV intasomes and deproteinized genomic sheep DNA. Alignment of the integration sites revealed symmetric sequence preferences that are consistent with integration of viral ends across 6 bp in target DNA in both conditions. As expected for a lentivirus, MVV displayed a strong preference for transcription units, with 70.2% integration sites found within predicted sheep genes, compared to 43.7% in the in vitro generated control condition.

ORGANISM(S): Ovis aries

PROVIDER: GSE87786 | GEO | 2017/01/10

SECONDARY ACCESSION(S): PRJNA347595

REPOSITORIES: GEO

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