Transcriptomics,Genomics

Dataset Information

158

Transcriptomic assessment of interactions between doublesex and its target genes across sexes and tissues in horned beetles


ABSTRACT: Purpose: Sexual dimorphisms fuel significant intraspecific variation and evolutionary diversification. Yet the developmental-genetic mechanisms underlying sex-specific development remain poorly understood. We focused on the conserved sex-determination gene doublesex (dsx) and the mechanisms by which it mediates sex-specific development in a species of horned beetle by combining systemic dsx knockdown, high-throughput sequencing of diverse tissues, and a genome-wide analysis of Dsx binding sites. We find that Dsx regulates sex-biased expression predominantly in males, that Dsx's target repertoires are highly sex- and tissue-specific, and that Dsx can exercise its regulatory role via two distinct mechanisms: as a sex-specific modulator by regulating strictly sex-specific targets, or as a switch by regulating the same genes in males and females in opposite directions. More generally, our results suggest Dsx can rapidly acquire new target gene repertoires to accommodate evolutionarily novel traits, evidenced by the large and unique repertoire identified in head horns, a recent morphological innovation. Overall design: Control and dsxRNAi individuals were collected within the first day after pupation. Six male and six female pupae from each group were sampled tissue for four distinct regions: the supraesophageal ganglion (brain), genitalia, thoracic horns, and head horns.

INSTRUMENT(S): Illumina NextSeq 500 (Onthophagus taurus)

SUBMITTER: Cristina Ledon-Rettig  

PROVIDER: GSE87788 | GEO | 2017-02-28

SECONDARY ACCESSION(S): PRJNA347620

REPOSITORIES: GEO

Similar Datasets

2010-09-15 | E-GEOD-23425 | ArrayExpress
2010-09-15 | GSE23425 | GEO
2007-11-06 | GSE9185 | GEO
2010-05-18 | E-GEOD-13616 | ArrayExpress
2009-06-01 | GSE13616 | GEO
2009-02-11 | GSE11311 | GEO
2009-02-10 | E-GEOD-11311 | ArrayExpress
2015-09-01 | E-GEOD-67400 | ArrayExpress
2016-06-01 | E-GEOD-80124 | ArrayExpress
| GSE67065 | GEO