Dataset Information


Comparison of Corynebacterium glutamicum chassis C1 with MB001

ABSTRACT: To identify genes which are differentially expressed in Corynebacterium glutamicum chassis C1 in comparison to the prophage free strain MB001, we performed DNA microarray analyses of C. glutamicum C1 against MB001. For this purpose RNA was isolated from cells cultivated in CGXII minimal medium with 2% glucose (w v-1) and harvested in the exponential growth phase at an OD600 of 5. Four biological replicates were performed. Overall design: Corynebacterium glutamicum chassis C1 in comparison to the prophage free strain MB001

INSTRUMENT(S): Agilent FZ_IBG1_4plex_064191

SUBMITTER: Tino Polen  

PROVIDER: GSE88717 | GEO | 2017-08-23



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Targeted top-down strategies for genome reduction are considered to have a high potential for providing robust basic strains for synthetic biology and industrial biotechnology. Recently, we created a library of 26 genome-reduced strains of Corynebacterium glutamicum carrying broad deletions in single gene clusters and showing wild-type-like biological fitness. Here, we proceeded with combinatorial deletions of these irrelevant gene clusters in two parallel orders, and the resulting library of 28  ...[more]

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