Polysome-seq in zebrafish early embryonic development

ABSTRACT: We followed the polysomal association of maternal and early zygotic transcriptome over the first few hours of embryonic development, prior to and after MBT. We isolated polysome-associated (bound) and non-polysome-associated (unbound) mRNAs using sucrose gradient centrifugation followed by size fractionation. Using next generation sequencing (RNA-seq), we profiled the transcriptome in polysome-bound and unbound fractions. Our analysis revealed distinct dynamics of polysome association of cytoplasmically polyadenylated maternal mRNAs.

ORGANISM(S): Danio rerio

PROVIDER: GSE88776 | GEO | 2017/10/14

SECONDARY ACCESSION(S): PRJNA348537

REPOSITORIES: GEO