Assessing characteristics of RNA amplification methods for single cell RNA sequencing

ABSTRACT: We conducted a large-scale control experiment to assess the transfer function of three scRNA-seq methods and factors modulating the function. Our approach was to dilute bulk total RNA (from a single source) to levels bracketing single-cell levels of total RNA (10 pg and 100 pg) in replicates and amplifying the RNA to levels sufficient for RNA sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE88850 | GEO | 2016/10/31

SECONDARY ACCESSION(S): PRJNA348767

REPOSITORIES: GEO